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Volume 4, Issue 4 (12-2018)
jhehp 2018, 4(4): 159-163 |
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Zaraatgar Gohardani H R, Moghanloo E, Badameh P, Rezaei S, Babaei V, Teimourian S. The Significant Association of the dupA and cagA genes of Helicobacter pylori with Peptic Ulcer. jhehp 2018; 4 (4) :159-163
URL: http://jhehp.zums.ac.ir/article-1-166-en.html
URL: http://jhehp.zums.ac.ir/article-1-166-en.html
Hamid Reza Zaraatgar Gohardani1 
, Ehsan Moghanloo2 , Parisa Badameh3 , Soheila Rezaei4 , Vahid Babaei5 , Shahram Teimourian *6
, Ehsan Moghanloo2 , Parisa Badameh3 , Soheila Rezaei4 , Vahid Babaei5 , Shahram Teimourian *6
1- Department of Basic Sciences, School of Microbiology Islamic Azad University, Damghan, Iran.
2- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
3- Department of Microbiology, College of Basic Science and Biology,Islamic Azad University Varamin, Iran.
4- Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
5- Department of Medical Genetics, Iran University of Medical Sciences,Tehran, Iran.
6- Department of Virology, Pasture Institute of Iran,Tehran, Iran.
2- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
3- Department of Microbiology, College of Basic Science and Biology,Islamic Azad University Varamin, Iran.
4- Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
5- Department of Medical Genetics, Iran University of Medical Sciences,Tehran, Iran.
6- Department of Virology, Pasture Institute of Iran,Tehran, Iran.
Abstract: (10809 Views)
Background: Helicobacter pylori play a significant etiological role in various digestive diseases. Peptic ulcer is caused by H. pylori, which destroys the duodenum mucus and is often observed in the individuals consuming tobacco, spicy and heavy meals, alcohol, coffee, and tranquilizers. Several studies have indicated that duodenal ulcer promoting genes dupA and cagA are involved in H. pylori etiology. The present study aimed to evaluate the correlation between these genes and peptic ulcer.
Methods: In this study, 500 stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations.
Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < 0.05). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection.
Conclusion: According to the results, there are significant correlations between tobacco use (P < 0.05), tranquilizer use (P < 0.05), and meteorism (P < 0.05) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with non-ulcer dyspepsia and peptic ulcer.
Methods: In this study, 500 stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations.
Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < 0.05). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection.
Conclusion: According to the results, there are significant correlations between tobacco use (P < 0.05), tranquilizer use (P < 0.05), and meteorism (P < 0.05) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with non-ulcer dyspepsia and peptic ulcer.
Type of Study: Original Article |
Subject:
Public Health
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/12/21
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/12/21
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