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Volume 4, Issue 4 (12-2018)
jhehp 2018, 4(4): 159-163 |
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Zaraatgar Gohardani H R, Moghanloo E, Badameh P, Rezaei S, Babaei V, Teimourian S. The Significant Association of the dupA and cagA genes of Helicobacter pylori with Peptic Ulcer. jhehp 2018; 4 (4) :159-163
URL: http://jhehp.zums.ac.ir/article-1-166-en.html
URL: http://jhehp.zums.ac.ir/article-1-166-en.html
Hamid Reza Zaraatgar Gohardani1 
, Ehsan Moghanloo2 , Parisa Badameh3 , Soheila Rezaei4 , Vahid Babaei5 , Shahram Teimourian *6
, Ehsan Moghanloo2 , Parisa Badameh3 , Soheila Rezaei4 , Vahid Babaei5 , Shahram Teimourian *6
1- Department of Basic Sciences, School of Microbiology Islamic Azad University, Damghan, Iran.
2- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
3- Department of Microbiology, College of Basic Science and Biology,Islamic Azad University Varamin, Iran.
4- Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
5- Department of Medical Genetics, Iran University of Medical Sciences,Tehran, Iran.
6- Department of Virology, Pasture Institute of Iran,Tehran, Iran.
2- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
3- Department of Microbiology, College of Basic Science and Biology,Islamic Azad University Varamin, Iran.
4- Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
5- Department of Medical Genetics, Iran University of Medical Sciences,Tehran, Iran.
6- Department of Virology, Pasture Institute of Iran,Tehran, Iran.
Abstract: (10792 Views)
Background: Helicobacter pylori play a significant etiological role in various digestive diseases. Peptic ulcer is caused by H. pylori, which destroys the duodenum mucus and is often observed in the individuals consuming tobacco, spicy and heavy meals, alcohol, coffee, and tranquilizers. Several studies have indicated that duodenal ulcer promoting genes dupA and cagA are involved in H. pylori etiology. The present study aimed to evaluate the correlation between these genes and peptic ulcer.
Methods: In this study, 500 stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations.
Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < 0.05). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection.
Conclusion: According to the results, there are significant correlations between tobacco use (P < 0.05), tranquilizer use (P < 0.05), and meteorism (P < 0.05) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with non-ulcer dyspepsia and peptic ulcer.
Methods: In this study, 500 stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations.
Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < 0.05). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection.
Conclusion: According to the results, there are significant correlations between tobacco use (P < 0.05), tranquilizer use (P < 0.05), and meteorism (P < 0.05) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with non-ulcer dyspepsia and peptic ulcer.
Type of Study: Original Article |
Subject:
Public Health
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/12/21
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/12/21
References
1. Momtaz H, Souod N, Dabiri H, Sarshar M. Study of Helicobacter pylori Genotype Status in Saliva, Dentalplaques, Stool and Gastric Biopsy Samples. World J Gastroenterol. 2012; 18: 2105-11. [Crossref]
2. Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin Microbiol Rev. 1997; 10: 720-41. [Crossref]
3. Kargar M, Souod N, Doosti A, Ghorbani-Dalini S. Prevalence of Helicobacter pylori Vacuolating Cytotoxin A Gene as a Predictive Marker for Different Gastrodoudenal Diseases. Iran J Clin Infect Dis. 2011; 6: 85-9.
4. Gerhard M, Lehn L, Neumaver N, Boren Th, Rad R, Schepp W, et al. Clinical Relevance of the Helicobacter pylori Gene for Blood Group Antigen-Binding Adhesion. Proc Natl Acad Sci USA. 1999; 96: 12778-83. [Crossref]
5. Abdulhamid M, Alkout C, Blackwell C, Donald M. Increased Inflammatory Responses of Persons of Blood Group O to Helicobacter pylori. J Infect Dis. 2000; 181: 1364-9. [Crossref]
6. Loffeld RJ, Stobberingh E. Helicobacter pylori and ABO Blood Groups. J Clin Pathol. 1991; 44: 516-7. [Crossref]
7. Momtaz H, Souod N, Dabiri H. Comparison of the Virulence Factors of Helicobacter pylori Isolated in Stomach and Saliva in Iran. Am J Med Sci. 2010; 340: 345-9. [Crossref]
8. Yamaoka Y. Roles of the Plasticity Regions of Helicobacter pylori in Gastroduodenal Pathogenesis. J Med Microbiol. 2008; 57: 545-53. [Crossref]
9. Matteo MJ, Armitano RI, Granados G, Wonaga AD, Sánches C, Olmos M, et al. Helicobacter pylori oipA, vacA and dupA Genetic Diversity in Individual Hosts. J Med Microbiol. 2010; 59: 89-95. [Crossref]
10. Massarrat S, Saberi-Firoozi M, Soleimani A, Himmelmann GW, Hitzges M, Keshavarz H. Peptic Ulcerdisease, Irritable Bowel Syndrome and Constipation in Two Populations in Iran. Eur J Gastroenterol Hepatol.1995; 7: 427-33.
11. Lu H, Hsu PI, Graham DY, Yamaoka Y. Duodenal Ulcer Promoting Gene of Helicobacter pylori. Gastroenterology. 2005; 128: 833-48. [Crossref]
12. Gomes LI, Rocha GA, Rocha AM, Soares TF, Oliveira CA, Bittencourt PF, et al. Lack of Association between Helicobacter pylori Infection with dupA-Positive Strains and Gastroduodenal Diseases in Brazilian Patients. Int J Med Microbiol. 2008; 298: 223-30. [Crossref]
13. Argent RH, Burette A, Miendje Deyi VY, Atherton JC. The Presence of dupA in Helicobacter pylori is Not Significantly Associated with Duodenal Ulceration in Belgium, South Africa, China, or North America. Clin Infect Dis. 2007; 45: 1204-6. [Crossref]
14. Kalra V, Lal B, Bhatia V, Baba CS, Chakravarthy S, Rohatgi S, Sarma PM, et al. Prevalence of Duodenal Ulcer-Promoting Gene (dupA) of Helicobacter pylori in Patients with Duodenal Ulcer in North Indian Population. Helicobacter. 2007; 12: 591-7. [Crossref]
15. Douraghi M, Mohammadi M, Oghalaie A, Abdirad A, Mohagheghi M, Eshagh Hosseini M, Zeraati H, et al. dupA as a Risk Determinant in Helicobacter pylori Infection. J Med Microbiol. 2008; 57: 554-62. [Crossref]
16. Pavlo S. ABO Lewis Secretor and Non Secretor Phenotypes Patients Infected by the Helico bacter pylory and Peptic Ulcer. J Clin Patho. 2001; 54: 809-11.
17. Holstege A. Effects of Nicotine, Alcohol and Caffeine on the Incidence, Healing and Recurrence Rate of Peptic Ulcer. Z Gastroenterol. 1987 ; 25: 33-40.
18. Aldoori WH, Giovannucci EL, Stampfer MJ, Rimm EB, Wing AL, Willett WC. A Prospective Study of Alcohol, Smoking, Caffeine and the Risk of Duodenal Ulcer in Men. Epidemiology. 1997; 8(4): 420-4. [Crossref]
19. Rosenstock S, Jørgensen T, Bonnevie O, Andersen L. Risk Factors for Peptic Ulcer Disease: A Population Based Prospective Cohort Study Comprising 2416 Danish Adults. Gut. 2003; 52(2): 186-93. [Crossref]
20. Wachirawat W, Hanucharurnkul S, Suriyawongpaisal P, Boonyapisit S, Levenstein S, Jearanaisilavong J, et al. Stress, but not Helicobacter pylori, is Associated with Peptic Ulcer Disease in a Thai Population. J Med Assoc Thai. 2003 ; 86: 672-85.
21. Al Mofleh IA. Spices, Herbal Xenobiotics and the Stomach: Friends or Foes? World J Gastroenterol. 2010; 16(22): 2710-9. [Crossref]
22. Herrera AG. Helicobacter pylori and Food Products: A Public Health Problem. Methods Mol Biol. 2004; 268: 297-301. [Crossref]
23. Seyda T, Derya C, Füsun A, Meliha K. The Relationship of Helicobacter pylori Positivity with Age, Sex, and ABO/Rhesus Blood Groups in Patients with Gastrointestinal Complaints in Turkey. Helicobacter. 2007; 12: 244-50. [Crossref]
24. Jaff MS. Relation between ABO Blood Groups and Helicobacter pylori Infection in Symptomatic Patients. Clin Exp Gastroenterol. 2011; 4: 221-6. [Crossref]
25. Yei CJ, Chang JG, Shih MC, Lin SF, Chang CS, Ko FT, et al. Lewis Blood Genotype of Peptic Ulcer and Gastric Cancer Patients in Taiwan. World J Gastroenterol. 2005; 11: 4891-4. [Crossref]
26. Shimamoto T, Yamamichi N, Kodashima S, Takahashi Y, Fujishiro M, OkaM, et al. No Association of Coffee Consumption with Gastric Ulcer, Duodenal Ulcer, Reflux Esophagitis, and Non-Erosive Reflux Disease: A Cross-Sectional Study of 8,013 Healthy Subjects in Japan. PLoS One. 2013; 8(6): e65996. [Crossref]
27. Ji KY, Hu FL. Interaction or Relationship between Helicobacter pylori and Non-Steroidal Anti-inflammatory Drugs in Upper Gastrointestinal Diseases. . World J Gastroenterol. 2006; 12: 3789-92. [Crossref]
28. Schmassmann A. Aspirin injury and H pylori. Gut. 2002; 50: 589-90. [Crossref]
29. Fletcher EH, Johnston DE, Fisher CR, Koerner RJ, Newton JL, Gray CS. Systematic Review: Helicobacter pylori and the Risk of Upper Gastrointestinal Bleeding Risk in Patients Taking Aspirin. Aliment Pharmacol Ther. 2010; 32: 831-9. [Crossref]
30. Shiota S, Matsunari O, Watada M, Hanada K, Yamaoka Y. Systematic Review and Meta-Analysis: the Relationship between the Helicobacter pylori dupA Gene and Clinical Outcomes. Gut Pathog. 2010; 2(1): 13-21. [Crossref]
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