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Volume 4, Issue 3 (9-2018)
jhehp 2018, 4(3): 138-143 |
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Moghanloo E, Ghorbani E, Beikverdi M S, Badameh P, Rezaei S, Piroozmand A, et al . The Netosis Formation of HL-60 Cell Differentiated to Neutrophil-Like Cells by LPS. jhehp 2018; 4 (3) :138-143
URL: http://jhehp.zums.ac.ir/article-1-165-en.html
URL: http://jhehp.zums.ac.ir/article-1-165-en.html
Ehsan Moghanloo1 
, Elham Ghorbani2 , Mohammad Sadegh Beikverdi3 , Parisa Badameh4 , Soheila Rezaei5 , Ahmad Piroozmand6 , Shahram Teimourian7 , Minoo Shahidi8 , Ahmad Khorshidi *1
, Elham Ghorbani2 , Mohammad Sadegh Beikverdi3 , Parisa Badameh4 , Soheila Rezaei5 , Ahmad Piroozmand6 , Shahram Teimourian7 , Minoo Shahidi8 , Ahmad Khorshidi *1
1- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
2- Department of Genetics Campus2, Guilan University of Medical Science, Rasht, Iran.
3- Department of Biology Islamic Azad University Central Tehran Branch, Tehran, Iran.
4- Department of Microbiology, College of Basic Science and Biology,Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
5- Department of Molecular Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
6- Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
7- Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran.
8- Department of Hematology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Genetics Campus2, Guilan University of Medical Science, Rasht, Iran.
3- Department of Biology Islamic Azad University Central Tehran Branch, Tehran, Iran.
4- Department of Microbiology, College of Basic Science and Biology,Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
5- Department of Molecular Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
6- Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
7- Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran.
8- Department of Hematology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract: (9269 Views)
Background: Neutrophils are the most abundant white blood cells in humans. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. NETs is a new strategy for pathogen response. This study focused on whether LPS induced NETs release in vitro in the HL60 cell line.
Methods: In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. Trypan blue as vital staining was used for viability. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA.
Results: Our results showed that 75% NETs was produced by HL-60 differentiated neutrophil cells exposed to 200 ng/ml LPS in 45 minues.
Conclusion: Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.
Methods: In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. Trypan blue as vital staining was used for viability. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA.
Results: Our results showed that 75% NETs was produced by HL-60 differentiated neutrophil cells exposed to 200 ng/ml LPS in 45 minues.
Conclusion: Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.
Type of Study: Original Article |
Subject:
Public Health
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/09/21
Received: 2018/08/11 | Accepted: 2018/09/8 | Published: 2018/09/21
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