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Department of Nursing, Faculty of Nursing, Egh.C., Islamic Azad University, Eghlid, Iran.
Abstract:   (107 Views)
Background: Airborne microorganisms in refrigerators may compromise the safety and quality of stored foods. This study assessed airborne bacterial and fungal contamination in dormitory and household refrigerators in Eghlid.
Methods: Airborne microorganisms were evaluated in 18 dormitory rooms and five household refrigerators using the passive settle plate method. Nutrient agar and Eosin Methylene Blue (EMB) were used for bacterial isolation, and Sabouraud Dextrose Agar (SDA) for fungal and yeast isolation. Plates were exposed inside refrigerators for 1 h and incubated at 35±2°C for bacteria and 25±2°C for fungi and yeasts. Microorganisms were identified using standard microscopic and biochemical methods. Air contamination levels were expressed as the Index of Microbial Air Contamination (IMA) (CFU/plate/h).
Results: Bacterial contamination exceeded fungal and yeast contamination in both refrigerator types. Staphylococcus aureus was the predominant bacterium, and Gram-positive bacteria were more prevalent. Among fungi, Aspergillus spp. (29%) and Penicillium spp. (26%) were the most prevalent genera, whereas Rhodotorula spp. (75%) was the predominant yeast. IMA was higher in dormitory refrigerators (26.39±11.66 CFU/plate/h) than in household refrigerators (12.0±4.53 CFU/plate/h) (Mann-Whitney U test, P = 0.01).
Conclusion: Airborne microbial contamination was detected in refrigerators, with higher levels in dormitory refrigerators than in household ones.

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Type of Study: Original Article | Subject: Environmental Health, Sciences, and Engineering
Received: 2026/04/4 | Accepted: 2026/06/10

References
1. Agi, V. N., Aleru, C. P., & Uweh, E. J. (2021). Bacterial contamination of some domestic and laboratory refrigerators in Port Harcourt Metropolis. European Journal of Health Sciences, 6(1), 16-34. [Crossref]
2. Alhassan, M., Nzeh, J., Quansah, L., & Dufailu, O. A. (2022). Antimicrobial resistance profile of Staphylococcus spp. and Listeria spp. isolated from refrigerators of retailers and students in the Tolon District, Ghana. Journal of Food Safety and Hygiene, 8(2), 122-131. [Crossref]
3. Altunatmaz, S. S., Issa, G., & Aydin, A. (2012). Detection of airborne psychrotrophic bacteria and fungi in food storage refrigerators. Brazilian Journal of Microbiology, 43(4), 1436-1443. [Crossref]
4. Düven, G., Tiryaki Gündüz, G., & Kışla, D. (2021). Determination of hygienic status of refrigerator surfaces. Food and Health, 7(4), 251-258. [Crossref]
5. Fazelipour, M., Neisi, A., Alizadehattar, S., & Kiasat, N. (2020). Isolation and identification of psychrotrophic fungal contamination in food storage refrigerators in Ahvaz city restaurants. Jundishapur Journal of Health Sciences, 12(2), 6-10. [Crossref]
6. Lalitha, C., Krishna, V. S., & Visakhapatnam, P. A. (2019). Contamination of refrigerator is a threat for infection. International Journal of Advanced Research, Ideas and Innovations in Technology, 5(2), 1514-1517.
7. Larone, D. H. (2011). Medically important fungi: A guide to identification (5th ed.). ASM Press. [Crossref]
8. MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria (3rd ed.). Lippincott Williams & Wilkins.
9. Mori, M., Sakagami, Y., Tanaka, M., Inoue, R., & Jojima, T. (2020). Analysis of the relationship of microbial contamination with temperature and cleaning frequency and method of domestic refrigerators in Japan. Journal of Food Protection, 83(7), 1234-1240. [Crossref]
10. Otu-Bassey, I. B., Ewaoche, I. S., Okon, B. F., & Ibor, U. A. (2017). Microbial contamination of house hold refrigerators in Calabar Metropolis-Nigeria. American Journal of Epidemiology and Infectious Disease, 5(1), 1-7.‌
11. Pasquarella, C., Pitzurra, O., & Savino, A. (2000). The index of microbial air contamination (IMA): A new index for the assessment of airborne microorganisms. Journal of Hospital Infection, 46(4), 241-256. [Crossref]
12. Sarabi, N., Sarkhosh, M., Najafpoor, A., Alidadi, H., & Baridkazemi, S. (2022). Investigation of bacterial and fungal contamination in domestic refrigerators. Journal of Research in Environmental Health, 8(3), 259-266.
13. Shahzad, A., Qureshi, A., Rehman, M. A. U., Ain, Q. U., Nawaz, A., Amir, A., . . . & Rizwan, M. (2025). Frequency and antimicrobial susceptibility pattern of bacteria and fungi isolated from household refrigerators. Frontiers in Medical and Health Research, 3(4), 428-445.
14. Soomro, R., Urooj, S., Ali Miran, Z., Huda, N., Naz, Sh., Mirbahar, A. A., . . . & Raza, Y. (2024). Illuminating biofilm formation and pathogen persistence in household refrigerators: Evidence from Karachi, Pakistan. Microbiological and Immunological Communications, 3(1), 81-92. [Crossref]
15. Taha, H. H., Abed, F. N. M., & Al-Rawi, J. M. (2025). Isolation and identification of fungal species contaminating refrigerators. Al-Kitab Journal for Pure Sciences, 9(1), 103-116. [Crossref]
16. Telli, A. E., Kahraman, H. A., Bicer, Y., & Gurbuz, U. (2019). Detection of the airborne microbiological contamination in domestic refrigerators in a student residential area of Konya, Turkey. Fresenius Environmental Bulletin, 28(6), 4790-4797.
17. Yong, S. S., Lee, J. I., & Kang, D. H. (2023). Bacterial composition of refrigerators in households and inactivation of airborne Staphylococcus aureus using a TiO₂ UVLED module in a 512 L aerobiology chamber. Food Microbiology, 114, 104274. [Crossref]
18. Zhang, W., Wang, J., Zhang, Y., Ye, K., Han, Y., Wang, C., . . . & Ge, X. (2022). Evaluation of bacterial contamination on three different surfaces within domestic refrigerators. Food Materials Research, 2(1), 1-7. [Crossref]

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