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Volume 7, Issue 1 (3-2021)
jhehp 2021, 7(1): 30-34 |
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Movafagh F, Zeinali T, Jamshidi A. Contamination Rate of Bovine Raw Milk with Yersinia enterocolitica Biotypes in Mashhad, Iran. jhehp 2021; 7 (1) :30-34
URL: http://jhehp.zums.ac.ir/article-1-398-en.html
URL: http://jhehp.zums.ac.ir/article-1-398-en.html
1- Graduated Student of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Social Determinants of Health Research Center, Department of Public Health, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Social Determinants of Health Research Center, Department of Public Health, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract: (5931 Views)
Background: Yersiniosis is an important foodborne zoonotic disease worldwide. Gastroenteritis and septicemia are the primary forms of yersiniosis. Yersinia enterocolitica is a causative organism, which is frequently isolated from foods such as milk. This study aimed to evaluate the contamination rate of bovine raw milk with Y. enterocolitica during January-July 2017 and the biotyping of the isolated organisms.
Methods: In total, 100 bovine raw milk samples were collected from Mashhad, Iran. The samples were enriched in peptone sorbitol broth. After alkali treatment, the enriched samples were streaked onto cefsulodin-irgasan-novobiocin agar plates. Suspected colonies of Y. enterocolitica were tested by the amplification of the 16s rRNA gene to confirm the isolates as Y. enterocolitica. The confirmed isolates were biotyped using biochemical tests.
Results: The results showed that 33% of the bovine raw milk samples were contaminated with Y. enterocolitica. Biotype 1A was predominant (26%), while the pathogenic biotypes 1B (6%) and 5 (1%) were also detected.
Conclusion: According to the results, the isolation of pathogenic Y. enterocolitica from bovine raw milk is a public health hazard due to its presence in dairy products.
Methods: In total, 100 bovine raw milk samples were collected from Mashhad, Iran. The samples were enriched in peptone sorbitol broth. After alkali treatment, the enriched samples were streaked onto cefsulodin-irgasan-novobiocin agar plates. Suspected colonies of Y. enterocolitica were tested by the amplification of the 16s rRNA gene to confirm the isolates as Y. enterocolitica. The confirmed isolates were biotyped using biochemical tests.
Results: The results showed that 33% of the bovine raw milk samples were contaminated with Y. enterocolitica. Biotype 1A was predominant (26%), while the pathogenic biotypes 1B (6%) and 5 (1%) were also detected.
Conclusion: According to the results, the isolation of pathogenic Y. enterocolitica from bovine raw milk is a public health hazard due to its presence in dairy products.
Type of Study: Original Article |
Subject:
Food Safety and Hygiene
Received: 2020/12/14 | Accepted: 2021/02/4 | Published: 2021/03/20
Received: 2020/12/14 | Accepted: 2021/02/4 | Published: 2021/03/20
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