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Volume 9, Issue 1 (3-2023)
jhehp 2023, 9(1): 12-19 |
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Dolatyari L, Gharavi Khiavi P, Yaftian M R. Development of a Dispersive Liquid-Liquid Microextraction for the Spectrophotometric Determination of Penicillin G in Milk and
Biological Samples. jhehp 2023; 9 (1) :12-19
URL: http://jhehp.zums.ac.ir/article-1-577-en.html
URL: http://jhehp.zums.ac.ir/article-1-577-en.html
1- Department of Chemistry, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
2- Department of Chemistry, Faculty of Science, The University of Zanjan, Zanjan, Iran.
2- Department of Chemistry, Faculty of Science, The University of Zanjan, Zanjan, Iran.
Abstract: (1793 Views)
Background: This study developed a dispersive liquid-liquid microextraction technique followed by spectrophotometric analysis for preconcentrating/determining penicillin G benzathine and penicillin G potassium.
Methods: The procedure was based on forming a color complex of penicillin G with bromothymol blue and its extraction by DLLME. The univariate method was employed for optimizing the parameters.
Results: Optimized conditions for extracting benzathine and potassium penicillin G were 1.5 mL methanol and acetone as dispersive solvents; 300 μL CHCl3 and 250 μL CCl4 as extracting solvents; pH 4 and 3; 0.8 mL and 0.9 mL bromothymol blue (10-3 M), and NaCl 0.8 M for both analytes. The calibration curves were linear over the ranges 50-750 and 800-950 IU mL-1 for analyzing benzathine, and 800-1600 and 1750-6500 IU mL-1 for potassium penicillin G. The relative standard deviations and detection limits were obtained to be 6.53% and 0.101 IU mL-1 and 4.65% and 0.149 IU mL-1 for determining benzathine and potassium penicillin G, respectively.
Conclusion: The proposed method was employed for extracting and determining penicillin in human plasma, urine, orange juice, and milk, with acceptable recovery values of 94.3-102%.
Methods: The procedure was based on forming a color complex of penicillin G with bromothymol blue and its extraction by DLLME. The univariate method was employed for optimizing the parameters.
Results: Optimized conditions for extracting benzathine and potassium penicillin G were 1.5 mL methanol and acetone as dispersive solvents; 300 μL CHCl3 and 250 μL CCl4 as extracting solvents; pH 4 and 3; 0.8 mL and 0.9 mL bromothymol blue (10-3 M), and NaCl 0.8 M for both analytes. The calibration curves were linear over the ranges 50-750 and 800-950 IU mL-1 for analyzing benzathine, and 800-1600 and 1750-6500 IU mL-1 for potassium penicillin G. The relative standard deviations and detection limits were obtained to be 6.53% and 0.101 IU mL-1 and 4.65% and 0.149 IU mL-1 for determining benzathine and potassium penicillin G, respectively.
Conclusion: The proposed method was employed for extracting and determining penicillin in human plasma, urine, orange juice, and milk, with acceptable recovery values of 94.3-102%.
Type of Study: Original Article |
Subject:
Environmental Health, Sciences, and Engineering
Received: 2022/12/28 | Accepted: 2023/02/23 | Published: 2023/03/11
Received: 2022/12/28 | Accepted: 2023/02/23 | Published: 2023/03/11
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