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Volume 8, Issue 3 (9-2022)
jhehp 2022, 8(3): 118-123 |
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Nwankwo E O, Nwagbara E, Okey-kalu E U. Plasmid Profile and Characterization of Escherichia coli Isolated from Diabetic Foot Ulcers in Imo State, South Eastern Nigeria. jhehp 2022; 8 (3) :118-123
URL: http://jhehp.zums.ac.ir/article-1-526-en.html
URL: http://jhehp.zums.ac.ir/article-1-526-en.html
1- Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B 7267, Umuahia, Abia State, Nigeria.
2- Department of Medical Laboratory Services, Savealife Mission Hospital, Onitsha, Anambra state, Nigeria.
2- Department of Medical Laboratory Services, Savealife Mission Hospital, Onitsha, Anambra state, Nigeria.
Abstract: (3446 Views)
Background: The study aimed at evaluating the plasmid profile and characterization of E. coli isolated from chronic diabetic foot ulcers among patients in Imo State, South East Nigeria.
Methods: A total of 150 wound swab samples were collected and analyzed. Standard microbiological methods processed samples. Antibiogram was done by Kirby-Bauer disc diffusion method. Tests were conducted to determine biofilm potential, ESBL and AmpC production. Plasmid profiling was done by the alkaline lysis method and Gel electrophoresis.
Results: Based on the results, out of the 150 swab samples, 61(40.6%) E. coli isolates were recovered. 57.8%, 32.8%, and 8.2% of the E. coli isolates were positive for biofilm formation, ESBL, and AmpC production, respectively. Further, the study revealed a pattern of multiple-drug resistance of E. coli to amoxicillin/clavulanic acid (96.7%), septrin (95.1%), and ampicillin (93.4%).
Conclusion: Analysis of the multiple antibiotic resistance (MAR) index showed that the organism had MAR index values above the low-risk region (≤0.2) for all the tested antibiotics. Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid-mediated. Findings could act as a therapeutic guide for antibiotic prescriptions in this environment.
Methods: A total of 150 wound swab samples were collected and analyzed. Standard microbiological methods processed samples. Antibiogram was done by Kirby-Bauer disc diffusion method. Tests were conducted to determine biofilm potential, ESBL and AmpC production. Plasmid profiling was done by the alkaline lysis method and Gel electrophoresis.
Results: Based on the results, out of the 150 swab samples, 61(40.6%) E. coli isolates were recovered. 57.8%, 32.8%, and 8.2% of the E. coli isolates were positive for biofilm formation, ESBL, and AmpC production, respectively. Further, the study revealed a pattern of multiple-drug resistance of E. coli to amoxicillin/clavulanic acid (96.7%), septrin (95.1%), and ampicillin (93.4%).
Conclusion: Analysis of the multiple antibiotic resistance (MAR) index showed that the organism had MAR index values above the low-risk region (≤0.2) for all the tested antibiotics. Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid-mediated. Findings could act as a therapeutic guide for antibiotic prescriptions in this environment.
Type of Study: Original Article |
Subject:
Environmental Health, Sciences, and Engineering
Received: 2022/06/25 | Accepted: 2022/08/9 | Published: 2022/09/19
Received: 2022/06/25 | Accepted: 2022/08/9 | Published: 2022/09/19
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