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Volume 7, Issue 4 (12-2021)
jhehp 2021, 7(4): 182-188 |
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Nouri P, Olfati A. The Effects of Maternal Exposure Riboflavin to Prevent Uterus Arsenic Damage in Offspring Rats. jhehp 2021; 7 (4) :182-188
URL: http://jhehp.zums.ac.ir/article-1-448-en.html
URL: http://jhehp.zums.ac.ir/article-1-448-en.html
1- Department of Midwifery, School of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2- Researcher, Young Researchers and Elites Club, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
2- Researcher, Young Researchers and Elites Club, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
Abstract: (4303 Views)
Background: In this study, the efficacy of riboflavin (VB2) in preventing uterus As2O3 damage was investigated for the first time in the literature.
Methods: The rats received 40 μg LHRHa for estrus synchronization. 48 pregnant Wistar rats were included in the study. Four groups were formed with 7 rats in each group: Sham, 1.5 mg arsenic trioxide (As2O3/L) alone or in combination with VB2 (20 and 40 mg/L) in drinking water (for 21 days continuously). Moreover, similar to maternal generation treatment the F1-female generation was arranged (for 35 days continuously until puberty).
Results: Based on the results, As2O3 reduced body weight and feed intake (P < 0.05). Furthermore, the serum malondialdehyde levels in the As2O3 group were significantly higher than that of the control group (P < 0.05). At the same time, the total antioxidative status and the activities of glutathione peroxidase, superoxide dismutase, and catalase were reduced (P < 0.05). Meanwhile, As2O3 remarkably increased the inflammatory markers production [interleukin 6 and C-reactive protein] (P < 0.05). As2O3 administration induced uterus apoptosis-related genes by upregulating caspase-3, iNOS, and Bax genes and downregulating Bcl-2 gene of pubertal F1-female rats (P < 0.05).
Conclusion: Our observation indicated that VB2 therapy is potentially an effective strategy to modify the detrimental effects of As2O3 in pubertal F1-female rats via suppresses oxidative damages.
Methods: The rats received 40 μg LHRHa for estrus synchronization. 48 pregnant Wistar rats were included in the study. Four groups were formed with 7 rats in each group: Sham, 1.5 mg arsenic trioxide (As2O3/L) alone or in combination with VB2 (20 and 40 mg/L) in drinking water (for 21 days continuously). Moreover, similar to maternal generation treatment the F1-female generation was arranged (for 35 days continuously until puberty).
Results: Based on the results, As2O3 reduced body weight and feed intake (P < 0.05). Furthermore, the serum malondialdehyde levels in the As2O3 group were significantly higher than that of the control group (P < 0.05). At the same time, the total antioxidative status and the activities of glutathione peroxidase, superoxide dismutase, and catalase were reduced (P < 0.05). Meanwhile, As2O3 remarkably increased the inflammatory markers production [interleukin 6 and C-reactive protein] (P < 0.05). As2O3 administration induced uterus apoptosis-related genes by upregulating caspase-3, iNOS, and Bax genes and downregulating Bcl-2 gene of pubertal F1-female rats (P < 0.05).
Conclusion: Our observation indicated that VB2 therapy is potentially an effective strategy to modify the detrimental effects of As2O3 in pubertal F1-female rats via suppresses oxidative damages.
Keywords: As2O3, Inflammation, Puberty, Vitamin B2
Type of Study: Original Article |
Subject:
Environmental Health, Sciences, and Engineering
Received: 2021/10/19 | Accepted: 2021/12/23 | Published: 2021/12/30
Received: 2021/10/19 | Accepted: 2021/12/23 | Published: 2021/12/30
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