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Volume 5, Issue 2 (6-2019)
jhehp 2019, 5(2): 79-85 |
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Hassanzadazar H, Yousefizadeh S, Ghafari A, Fathollahi M, Aminzare M. Antimicrobial Effects of the Nanoemulsion of Rosemary Essential Oil against Important Foodborne Pathogens. jhehp 2019; 5 (2) :79-85
URL: http://jhehp.zums.ac.ir/article-1-223-en.html
URL: http://jhehp.zums.ac.ir/article-1-223-en.html
Hassan Hassanzadazar *1 
, Samira Yousefizadeh2 , Azadeh Ghafari3 , Mehdi Fathollahi1 , Majid Aminzare1
, Samira Yousefizadeh2 , Azadeh Ghafari3 , Mehdi Fathollahi1 , Majid Aminzare1
1- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Science, Zanjan, Iran.
2- Student Research Committee, School of Public Health, Zanjan University of Medical Science, Zanjan, Iran.
3- Department of Food and Drug Control, School of Pharmacy, Zanjan University of Medical Science, Zanjan, Iran.
2- Student Research Committee, School of Public Health, Zanjan University of Medical Science, Zanjan, Iran.
3- Department of Food and Drug Control, School of Pharmacy, Zanjan University of Medical Science, Zanjan, Iran.
Abstract: (9233 Views)
Background: The purpose of this study was to determine the effect of rosemary essential oil (REO) nanoemulsion against some important food borne pathogens.
Methods: Antibacterial effects of REO and REO nanoemulsion were determined using Agar disc diffusion, Broth microdilution and Steam phase diffusion methods against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shewanella SP, Listeria monocytogenes and Salmonella enteritidis.
Results: Antibacterial effect of REO and REO nanoemulsion was increased with concentration enhancing of REO. There was no significant antibacterial activity in the effectiveness of nanoemulsion on the studied bacteria in comparison with REO in both disk diffusion and steam phase diffusion methods. MIC and MBC analysis of REO and prepared REO nanoemulsion showed that REO and its nanoemulsion have inhibited all studied bacteria. REO showed better inhibitory effects. REO and nanoemulsion of rosemary essential oil have the greatest effect on Shewanella SP., L. monocytogenes, S. aureus, S.enteritidis, E. coli and P. aeruginosa, respectively.
Conclusion: In total, it can be said that REO and its nanoemulsion are desirable to inhibit the growth of food borne pathogens and can be a good choice as antimicrobial agents in food industry to enhance safety and extend foods’ shelf life.
Methods: Antibacterial effects of REO and REO nanoemulsion were determined using Agar disc diffusion, Broth microdilution and Steam phase diffusion methods against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shewanella SP, Listeria monocytogenes and Salmonella enteritidis.
Results: Antibacterial effect of REO and REO nanoemulsion was increased with concentration enhancing of REO. There was no significant antibacterial activity in the effectiveness of nanoemulsion on the studied bacteria in comparison with REO in both disk diffusion and steam phase diffusion methods. MIC and MBC analysis of REO and prepared REO nanoemulsion showed that REO and its nanoemulsion have inhibited all studied bacteria. REO showed better inhibitory effects. REO and nanoemulsion of rosemary essential oil have the greatest effect on Shewanella SP., L. monocytogenes, S. aureus, S.enteritidis, E. coli and P. aeruginosa, respectively.
Conclusion: In total, it can be said that REO and its nanoemulsion are desirable to inhibit the growth of food borne pathogens and can be a good choice as antimicrobial agents in food industry to enhance safety and extend foods’ shelf life.
Keywords: Agar disk diffusion test, Broth microdilution method, Nanoemulsion, Rosemary essential oil, Steam phase diffusion method
Type of Study: Original Article |
Subject:
Food Safety and Hygiene
Received: 2019/02/26 | Accepted: 2019/05/30 | Published: 2019/06/30
Received: 2019/02/26 | Accepted: 2019/05/30 | Published: 2019/06/30
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