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1- Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Abstract: (47 Views)
Background: Rainbow trout (Oncorhynchus mykiss), an economically important fish in Iran, is valued for its nutritional potential. Aeromonas hydrophila causes infections that lead to motile septicaemia in fish and humans worldwide. Given the rapid expansion of fish farming in Iran, particularly in Zanjan Province, and the associated losses due to bacterial infections, this study aimed to detect A. hydrophila in farmed rainbow trout using molecular methods.
Methods: A total of 103 fish samples were collected from ponds across five cities in Zanjan Province. Cultured specimens were subsequently analyzed, and A. hydrophila isolates were confirmed through standard biochemical tests. Genomic DNA was extracted from the isolates, and the aerA and lip genes were identified as markers for confirmation of A. hydrophila using the PCR method.
Results: Overall, 72 (69.90%) samples were positive for A. hydrophila, as determined by both culture and biochemical tests. However, molecular analysis confirmed the aerA gene in 66 (64.07%) of the isolates, while only 3 (2.91%) samples carried both the lip and aerA genes.
Conclusion: The high prevalence of A. hydrophila in rainbow trout poses a threat to public health and results in significant economic losses in aquaculture. Collaboration among epidemiologists, microbiologists, veterinarians, and food safety experts is essential to reduce these impacts.
Methods: A total of 103 fish samples were collected from ponds across five cities in Zanjan Province. Cultured specimens were subsequently analyzed, and A. hydrophila isolates were confirmed through standard biochemical tests. Genomic DNA was extracted from the isolates, and the aerA and lip genes were identified as markers for confirmation of A. hydrophila using the PCR method.
Results: Overall, 72 (69.90%) samples were positive for A. hydrophila, as determined by both culture and biochemical tests. However, molecular analysis confirmed the aerA gene in 66 (64.07%) of the isolates, while only 3 (2.91%) samples carried both the lip and aerA genes.
Conclusion: The high prevalence of A. hydrophila in rainbow trout poses a threat to public health and results in significant economic losses in aquaculture. Collaboration among epidemiologists, microbiologists, veterinarians, and food safety experts is essential to reduce these impacts.
Type of Study: Original Article |
Subject:
Food Safety and Hygiene
Received: 2025/09/20 | Accepted: 2025/11/22
Received: 2025/09/20 | Accepted: 2025/11/22
References
1. Adanır, D., & Turutoglu, H. (2007). Isolation and antibiotic susceptibility of Aeromonas hydrophila in a carp (Cyprinus carpio) hatchery farm. Bulletin of the Veterinary Institute in Puławy, 51(3), 361-364.
2. Ahangarzadeh, M., Ghorbanpour, M., Peyghan, R., Sharif, R. M., & Soltani, M. (2015). Role of Aeromonas hydrophila in bacterial septicemia of cultured carps in khouzestan province. Iranian Veterinary Journal, 11(3), 5-16.
3. Ahangarzadeh, M., Ghorbanpour Najafabadi, M., Peyghan, R., Houshmand, H., Rohani, M. S., & Soltani, M. (2022). Detection and distribution of virulence genes in Aeromonas hydrophila isolates causing infection in cultured carps. Veterinary Research Forum, 13(1), 55-60.
4. Ahmed, H. A., Mohamed, M. E., Rezk, M. M., Gharieb, R. M., & Abdel-Maksoud, S. A. (2018). Aeromonas hydrophila in fish and humans; Prevalence, virulotyping and antimicrobial resistance. Slovenian Veterinary Research, 55(20), 113-124. [Crossref]
5. Cascón, A., Anguita, J., Hernanz, C., Sánchez, M., Fernandez, M., & Naharro, G. (1996). Identification of Aeromonas hydrophila hybridization group 1 by PCR assays. Applied and Environmental Microbiology, 62(4), 1167-1170. [Crossref]
6. Choresca Jr, C. H., Gomez, D. K., Han, J. E., Shin, S. P., Kim, J. H., Jun, J. W., & Park, S. C. (2010). Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium. Korean Journal of Veterinary Research, 50(4), 331-333.
7. Christy, G., Kusdawarti, R., & Handijatno, D. (2019). Determination of the aerolysin gene in Aeromonas hydrophila using the polymerase chain reaction (PCR) technique. IOP Conference Series: Earth and Environmental Science, 236, 012097. [Crossref]
8. Citterio, B., & Biavasco, F. (2015). Aeromonas hydrophila virulence. Virulence, 6(5), 417-418. [Crossref]
9. Daneshamouz, Sh., Haghi, F., & Zeighami, H. (2020). Detection and identification of bacterial pathogens in rainbow trout (Oncorhynchus mykiss) samples from fish farms in Iran. Thalassas: An International Journal of Marine Sciences, 36(1), 133-141. [Crossref]
10. Fadaeifard, F. (2014). Molecular detection of Aeromonas hydrophila in the aquarium gold fish and cultured rainbow trout in Chaharmahal va Bakhtiary province. Veterinary Clinical Pathology, 8(1), 401-409.
11. Janda, J. M., & Abbott, S. L. (2010). The genus Aeromonas: Taxonomy, pathogenicity, and infection. Clinical Microbiology Reviews, 23(1), 35-73. [Crossref]
12. Khamesipour, F., Moradi, M., Noshadi, E., & Momeni Shahraki, M. (2014). Detection of the prevalence of Aeromonas hyrophila in shrimp samples by polymerase chain reaction (PCR) and cultural method in the Iran. Journal of Biodiversity and Environmental Sciences, 4(2), 47-52.
13. Moradi, F., Hadi, N., Bazargani, A., Abdi, F., & Ghorbanian, N. (2025). Aeromon:as char:acteristics in Iran, Southwest Asia; A systematic review and meta-analysis on epidemiology, reservoirs and antibiotic resistance profile from aquatic environments to human society during 2000-2023. BMC Veterinary Research, 21(1), 107. [Crossref]
14. Pakravan, S., Hajimoradloo, A., & Ghorbani, R. (2012). Effect of dietary willow herb, Epilobium hirsutum extract on growth performance, body composition, haematological parameters and Aeromonas hydrophila challenge on common carp, Cyprinus carpio. Aquaculture Research, 43(6), 861-869. [Crossref]
15. Patil, R. D., Bisht, E., Verma, A., Sharma, R., & Asrani, R. K. (2025). Pathology of Aeromonas hydrophila infection in rainbow trout (On-corhynchus mykiss) of Himachal Pradesh. Indian Journal of Veterinary Pathology, 49(1), 85-89. [Crossref]
16. Perry, S. F., & Laurent, P. (1993). Environmental effects on fish gill structure and function. In J. C. Rankin, & F. B. Jensen, Fish ecophysiology (pp. 231-264). Springer. [Crossref]
17. Rasmussen-Ivey, C. R., Figueras, M. J., McGarey, D., & Liles, M. R. (2016). Virulence factors of Aeromonas hydrophila: In the wake of reclassification. Frontiers in Microbiology, 7, 01337. [Crossref]
18. Saad, T., & Atallah, S. (2015). Aeromonas hydrophilla in cultured oreochromis niloticus and its effect on economic returne of fish farm production. International Journal on Recent Trends in Life Science and Mathematics, 2(4), 15-19.
19. Sahoo, P. K., Rauta, P. R., Mohanty, B. R., Mahapatra, K. D., Saha, J. N., Rye, M., & Eknath, A. E. (2011). Selection for improved resistance to Aeromonas hydrophila in Indian major carp Labeo rohita: Survival and innate immune responses in first generation of resistant and susceptible lines. Fish & Shellfish Immunology, 31(3), 432-438. [Crossref]
20. Sheikh, H. I., Nordin, B., Paharuddin, N., Liew, H. J., Fadhlina, A., Abdulrazzak, L. A., . . . & Musa, N. (2023). Virulence factors and mechanisms of Aeromonas hydrophila infection in catfish Siluriformes: A review and bibliometric analysis. Desalination and Water Treatment, 315, 538-547. [Crossref]
21. Swaminathan, T. R., Rathore, G., Abidi, R., & Kapoor, D. (2004). Detection of Aeromonas hydrophila by polymerase chain reaction. Indian Journal of Fisheries, 51(2), 251-254.
22. Tolouei Gilani, J., Goudarztalejerdi, A., Yavari, M., & Nouri Kalourazi, M. (2021). Isolation and identification of Aeromonas hydrophila from Cyprinidae suspected with hemorrhagic septicemia in pools of warm water fishes in Gilan Province. International Journal of Nutrition Sciences, 6(1), 52-58.
23. Uma, A., Rebecca, G., Meena, S., & Saravanabava, K. (2010). PCR detection of putative aerolysin and hemolysin genes in an Aeromonas hydrophila isolate from infected Koi carp (Cyprinus carpio). Tamilnadu Journal of Veterinary and Animal Sciences, 6(1), 31-33.
24. Vega-Sánchez, V., Acosta-Dibarrat, J., Vega-Castillo, F., Castro-Escarpulli, G., Aguilera-Arreola, M. G., & Soriano-Vargas, E. (2014). Phenotypical characteristics, genetic identification, and antimicrobial sensitivity of Aeromonas species isolated from farmed rainbow trout (Onchorynchus mykiss) in Mexico. Acta Tropica, 130, 76-79. [Crossref]
25. Vivas, J., Razquin, B., Lopez-Fierro, P., & Villena, A. J. (2005). Modulation of the immune response to an Aeromonas hydrophila aroA live vaccine in rainbow trout: Effect of culture media on the humoral immune response and complement consumption. Fish & Shellfish Immunology, 18(3), 223-233. [Crossref]
26. Wang, G., Clark, C. G., Liu, C., Pucknell, C., Munro, C. K., Kruk, T. M., . . . & Rodgers, F. G. (2003). Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. Journal of Clinical Microbiology, 41(3), 1048-1054. [Crossref]
27. Wang, Y., Tang, C., Yu, X., Wang, Y., & Yue, H. (2008). Detecting pathogenic Aeromonas hydrophila in fish by triplex PCR. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 48(7), 947-951.
28. Zorriehzahra, M. J., Yazdanpanah-Goharrizi, L., Rokhbakhsh-Zamin, F., Kazemipour, N., & Kheirkhah, B. (2020). Isolation, biochemical and molecular detection of Aeromonas hydrophila from cultured Oncorhynchus mykiss. Iranian Journal of Fisheries Sciences, 19(5), 2422-2436. [Crossref]
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