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1- Department of Environmental Health, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
2- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
2- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
Abstract: (99 Views)
Background: In recent years, the use of antimicrobial properties of nanoparticles has received much attention due to the resistance of bacteria to antibiotics.
Methods: This study achieved the green synthesis of silver nanoparticles (AgNPs) using Agrimonia eupatoria L. extract, and evaluated their physicochemical properties using UV-Vis spectrophotometry, DLS, FT-IR, FESEM, and EDAX and antimicrobial activity by MIC and MBC values.
Results: DLS analysis revealed average sizes of 72.8 nm and 124.8 nm for green and chemically synthesized AgNPs, respectively. FESEM images showed spherical shapes, and EDAX confirmed the presence of silver, carbon, and oxygen on the nanoparticles. Antimicrobial testing (MIC and MBC) showed that green-synthesized AgNPs exhibited stronger antibacterial activity than chemically synthesized ones. For Escherichia coli, the MIC and MBC for green-synthesized AgNPs were 0.028 and 0.056 mg/ml, respectively, compared to 0.125 and 0.25 mg/ml for chemically synthesized AgNPs. Similarly, for Staphylococcus aureus, the green-synthesized nanoparticles had MIC and MBC values of 0.226 mg/ml, while chemically synthesized nanoparticles had 0.125 and 0.25 mg/ml.
Conclusion: These results demonstrated higher antimicrobial potential of plant-mediated silver nanoparticle synthesis to chemical methods, especially against Gram-negative bacteria.
Methods: This study achieved the green synthesis of silver nanoparticles (AgNPs) using Agrimonia eupatoria L. extract, and evaluated their physicochemical properties using UV-Vis spectrophotometry, DLS, FT-IR, FESEM, and EDAX and antimicrobial activity by MIC and MBC values.
Results: DLS analysis revealed average sizes of 72.8 nm and 124.8 nm for green and chemically synthesized AgNPs, respectively. FESEM images showed spherical shapes, and EDAX confirmed the presence of silver, carbon, and oxygen on the nanoparticles. Antimicrobial testing (MIC and MBC) showed that green-synthesized AgNPs exhibited stronger antibacterial activity than chemically synthesized ones. For Escherichia coli, the MIC and MBC for green-synthesized AgNPs were 0.028 and 0.056 mg/ml, respectively, compared to 0.125 and 0.25 mg/ml for chemically synthesized AgNPs. Similarly, for Staphylococcus aureus, the green-synthesized nanoparticles had MIC and MBC values of 0.226 mg/ml, while chemically synthesized nanoparticles had 0.125 and 0.25 mg/ml.
Conclusion: These results demonstrated higher antimicrobial potential of plant-mediated silver nanoparticle synthesis to chemical methods, especially against Gram-negative bacteria.
Type of Study: Original Article |
Subject:
Environmental Health, Sciences, and Engineering
Received: 2025/01/21 | Accepted: 2025/05/10
Received: 2025/01/21 | Accepted: 2025/05/10
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