Volume 8, Issue 2 (6-2022)                   jhehp 2022, 8(2): 110-117 | Back to browse issues page


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Yousefizadeh S, Aminzare M, hassanzadazar H. Synergistic Antioxidant and Antimicrobial Effects of the Thymoquinone and Eugenol Combination. jhehp 2022; 8 (2) :110-117
URL: http://jhehp.zums.ac.ir/article-1-520-en.html
1- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (3625 Views)
Background: The present study aimed to evaluate the in-vitro antioxidant and antimicrobial activity of Thymoquinone (TQ), Eugenol (EUG) and their synergistic effects in combination.
Materials: The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration (FIC), and disc diffusion tests were used to determine the antibacterial activity of TQ and EUG.  Moreover, Reducing Power Assay and DPPH (2.2-diphenyl-2-picrylhydrazyl) tests were used to indicate the antioxidant ability of TQ and EUG.  The combination index (CI) of these compounds was calculated to evaluate their interaction type.
Results: Bacillus cereus, Listeria monocytogenes, and Shewanella SP were the most sensitive bacteria than other studied pathogens to TQ and EUG, with MIC 0.019, 0.07, and 0.07 mg/ml for TQ and 0.6, 0.6, and 0.6 mg/ml for EUG, respectively.  The mixture of two ingredients showed a good antimicrobial and synergistic effects against all tested bacteria. The DPPH scavenging activity of the combination of TQ+ EUG were antagonistic in all combined concentrations except for TQ (5 mg/ml) + EUG (1.25 mg/ml) and TQ (2.5 mg/ml) + EUG (0.3 mg/ml) treatments.
Conclusion: The obtained results provided a new combination of antimicrobial and antioxidant agents in drug delivery, especially in food preservation systems, to enhance food shelf life in the food industry.
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Type of Study: Original Article | Subject: Food Safety and Hygiene
Received: 2022/03/15 | Accepted: 2022/05/18 | Published: 2022/06/18

References
1. Rodriguez-Garcia I, Silva-Espinoza BA, Ortega-Ramirez LA, Leyva JM, Siddiqui MW, Cruz-Valenzuela MR, Gonzalez-Aguilar GA, Ayala-Zavala JF. Oregano Essential Oil as an Antimicrobial and Antioxidant Additive in Food Products. Crit Rev Food Sci Nutr. 2016; 56(10): 1717-27. [Crossref] [Google Scholar]
2. Ribeiro-Santos R, Andrade M, Sanches-Silva A, De Melo NR. Essential Oils for Food Application: Natural Substances with Established Biological Activities. Food Bioproc Tech. 2018; 11(1): 43-71. [Crossref] [Google Scholar]
3. Bintsis T. Foodborne Pathogens. AIMS Microbiol. 2017; 3(3): 529-63. [Crossref] [PubMed]
4. Tippayatum P, Chonhenchob V. Antibacterial Activities of Thymol, Eugenol and Nisin Against Some Food Spoilage Bacteria. Nat Sci. 2007; 41:319-23. [Google Scholar]
5. Xu T, Gao C, Feng X, Yang Y, Shen X, Tang X. Structure, Physical and Antioxidant Properties of Chitosan-Gum Arabic Edible Films Incorporated with Cinnamon Essential Oil. Int J Biol Macromol. 2019; 134: 230-6. [Crossref] [Google Scholar]
6. Al Turkmani MO, Karabet F, Mokrani L , Soukkarieh C. Chemical Composition and in Vitro Antioxidant Activities of Essential Oil from Nigella Sativa L. Seeds Cultivated in Syria. Int J Chemtech Res. 2015; 8(10): 76-82. [Google Scholar]
7. Singh S, Das SS, Singh G, Schuff C, De Lampasona MP, Catalan CA. Composition, in Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained from Black Cumin Seeds (Nigella Sativa L.). Biomed Res Int. 2014; 2014: 1-10. [Crossref] [Google Scholar]
8. Badawy ME, Marei GI, Rabea EI, Taktak NE. Antimicrobial and Antioxidant Activities of Hydrocarbon and Oxygenated Monoterpenes Against Some Food-Borne Pathogens Through in Vitro and in Silico Studies. Pestic Biochem Phys. 2019; 158: 185-200. [Crossref] [Google Scholar]
9. Yimer EM, Tuem KB, Karim A, Ur-Rehman N, Anwar F. Nigella Sativa L. (Black Cumin): A Promising Natural Remedy for Wide Range of Illnesses. Evid Based Complement Alternat Med. 2019; 2019: 1-16. [Crossref] [Google Scholar]
10. Liu Q, Niu H, Zhang W, Mu H, Sun C, Duan J. Synergy Among Thymol, Eugenol, Berberine, Cinnamaldehyde and Streptomycin Against Planktonic and Biofilm‐Associated Food‐Borne Pathogens. Lett Appl Microbiol. 2015; 60(5): 421-30. [Crossref] [Google Scholar]
11. Nallamuthu I, Parthasarathi A, Khanum F. Thymoquinone-Loaded Plga Nanoparticles: Antioxidant and Antimicrobial Properties. Int J Curr Pharm Res. 2013; 2(12): 202-7. [Crossref] [Google Scholar]
12. Mohammadabadi MR, Mozafari MR. Enhanced Efficacy and Bioavailability of Thymoquinone Using Nanoliposomal Dosage form. J Drug Deliv Sci Technol. 2018; 47: 445-53. [Crossref] [Google Scholar]
13. Gülçin İ. Antioxidant Activity of Eugenol: A Structure–Activity Relationship Study. J Med Food. 2011; 14(9): 975-85. [Crossref] [Google Scholar]
14. Barboza JN, Da Silva Maia Bezerra Filho C, Silva RO, Medeiros JV, De Sousa DP. An Overview on the Anti-Inflammatory Potential and Antioxidant Profile of Eugenol. Oxid Med Cell Longev. 2018; 2018: 3957262. [Crossref] [Google Scholar]
15. Zhang LL, Zhang LF, Xu JG, Hu QP. Comparison Study on Antioxidant, DNA Damage Protective and Antibacterial Activities of Eugenol and Isoeugenol Against Several Food-Borne Pathogens. Food Nutr Res. 2017; 61(1): 1353356. [Crossref] [Google Scholar]
16. Han X, Parker TL. Anti-inflammatory Activity of Clove (Eugenia Caryophyllata) Essential Oil in Human Dermal Fibroblasts. Pharm Biol. 2017; 55(1): 1619-22. [Crossref] [Google Scholar]
17. Soufi Sh, Aminzare M, Hassanzadazar H, Kamali K. Comparative Study on the Antibacterial Properties of Nanoemulsion of Zataria Multiflora Essential Oil Fortified with Cinnamaldehyde Against Common Foodborne Pathogens. The Natural Products J. 2020; 10: 1-11. [Crossref] [Google Scholar]
18. Randhawa MA, Alenazy AK, Alrowaili MG, Basha J. An Active Principle of Nigella Sativa L, Thymoquinone, Showing Significant Antimicrobial Activity Against Anaerobic Bacteria. J Intercult Ethnopharmacol. 2017; 6(1): 97-101. [Crossref] [Google Scholar]
19. Fathollahi M, Aminzare M, Mohseni M, Hassanzadazar H. Antioxidant Capacity, Antimicrobial Activities and Chemical Composition of Pistacia Atlantica Subsp. Kurdica Essential Oil. Vet Res Forum. 2019; 10(4): 299-305. [Google Scholar]
20. Hassanzadazar H, Yousefizadeh S, Ghafari A, Fathollahi M, Aminzare M. Antimicrobial Effects of the Nanoemulsion of Rosemary Essential Oil Against Important Food-Borne Pathogens. J Hum Environ Health Promot. 2019; 5(2): 79-85. [Crossref] [Google Scholar]
21. Sharma G, Raturi K, Dang S, Gupta S, Gabrani R. Inhibitory Effect of Cinnamaldehyde Alone and in Combination with Thymol, Eugenol and Thymoquinone Against Staphylococcus Epidermidis. J Herb Med. 2017; 9: 68-73. [Crossref] [Google Scholar]
22. Sharma K, Guleria S, Razdan VK, Babu V. Synergistic Antioxidant and Antimicrobial Activities of Essential Oils of Some Selected Medicinal Plants in Combination and with Synthetic Compounds. Ind Crops Prod. 2020; 154: 112569. [Crossref] [Google Scholar]
23. Kadri A, Gharsallah N, Damak M, Gdoura R. Chemical Composition and in Vitro Antioxidant Properties of Essential Oil of Ricinus Communis L. J Med Plants Res. 2011; 5(8): 1466-70. [Google Scholar]
24. Mercado-Mercado G, Laura A, Alvarez-Parrilla E. Effect of Pectin on the Interactions Among Phenolic Compounds Determined by Antioxidant Capacity. J Mol Struct. 2020; 1199: 126967. [Crossref] [Google Scholar]
25. Muhammad DR, Praseptiangga D, Van de Walle D, Dewettinck K. Interaction Between Natural Antioxidants Derived from Cinnamon and Cocoa in Binary and Complex Mixtures. Food Chem. 2017; 231: 356-64. [Crossref] [Google Scholar]
26. Kouidhi B, Zmantar T, Jrah H, Souiden Y, Chaieb K, Mahdouani K, Bakhrouf A. Antibacterial and Resistance-Modifying Activities of Thymoquinone Against Oral Pathogens. Ann Clin Microbiol Antimicrob. 2011; 10(1): 29. [Crossref] [Google Scholar]
27. Halawani E. Antibacterial Activity of Thymoquinone and Thymohydroquinone of Nigella Sativa L. and their Interaction with Some Antibiotics. Adv Biol Res. 2009; 3(5-6): 148-52. [PubMed]
28. Kokoska L, Havlik J, Valterova I, Sovova H, Sajfrtova M, Jankovska I. Comparison of Chemical Composition and Antibacterial Activity of Nigella Sativa Seed Essential Oils Obtained by Different Extraction Methods. J Food Prot. 2008; 71(12): 2475-80. [Crossref] [PubMed]
29. Goel S, Mishra P. Thymoquinone Inhibits Biofilm Formation and has Selective Antibacterial Activity Due to ROS Generation. Appl Microbiol Biotechnol. 2018; 102(4): 1955-67. [Crossref] [Google Scholar]
30. El Atki Y, Aouam I, El Kamari F, Taroq A, Gourch A, Lyoussi B, Abdellaoui A. Antibacterial Efficacy of Thymol, Carvacrol, Eugenol and Menthol as Alternative Agents to Control the Growth of Nosocomial Infection-Bacteria. J Pharm Sci Res. 2019; 11(2): 306-9. [Google Scholar]
31. Catherine AA, Deepika H, Negi PS. Antibacterial Activity of Eugenol and Peppermint Oil in Model Food Systems. J Essent Oil Res. 2012; 24(5): 481-6. [Crossref] [Google Scholar]
32. Pei RS, Zhou F, Ji BP, Xu J. Evaluation of Combined Antibacterial Effects of Eugenol, Cinnamaldehyde, Thymol, and Carvacrol Against E. coli with an Improved Method. J Food Sci. 2009; 74(7): 379-83. [Crossref] [Google Scholar]
33. Theanphong O, Mingvanish W, Jenjittikul T. Chemical Constituents and in Vitro Antioxidant Activities of Essential Oil from Curcuma Leucorrhiza Roxb. Rhizome. Inter Prof J Health Sci. 2016; 14(2): 86-96. [Google Scholar]
34. Bakari S, Ncir M, Felhi S, Hajlaoui H, Saoudi M, Gharsallah N, Kadri A. Chemical Composition and in Vitro Evaluation of Total Phenolic, Flavonoid, and Antioxidant Properties of Essential Oil and Solvent Extract from the Aerial Parts of Teucrium Polium Grown in Tunisia. Food Sci Biotechnol. 2015; 24(6):1943-9. [Crossref] [Google Scholar]
35. Barhouchi B, Aouadi S, Abdi A. Determination of Eugenol and its Derivative Isoeugenol in Globularia Alypum Using Solvent System Extraction and Comparative Study of their Antioxidant Activities with Various Oxidation Conditions. J Chem Pharm Res. 2014; 6: 776-84. [Google Scholar]
36. Khither H, Sobhi W, Khenchouche A, Mosbah A, Benboubetra M. In-vitro Antioxidant Effect of Thymoquinone. Annu Res Rev Biol. 2018; 25(5): 1-9. [Crossref] [Google Scholar]
37. Butt AS, Nisar N, Mughal TA, Ghani N, Altaf I. Anti-Oxidative and Anti-Proliferative Activities of Extracted Phytochemical Compound Thymoquinone. J Pak Med Assoc. 2019; 69(10): 1479. [Crossref] [Google Scholar]
38. Mahapatra SK, Roy S. Phytopharmacological Approach of Free Radical Scavenging and Anti-Oxidative Potential of Eugenol and Ocimum Gratissimum Linn. Asian Pac J Trop Med. 2014; 7: S391-7. [Crossref] [Google Scholar]
39. Olszowy-Tomczyk M. Synergistic, Antagonistic and Additive Antioxidant Effects in the Binary Mixtures. Phytochem Rev. 2020; 19: 63–103. [Crossref] [Google Scholar]

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