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Volume 7, Issue 1 (3-2021)
jhehp 2021, 7(1): 30-34 |
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Movafagh F, Zeinali T, Jamshidi A. Contamination Rate of Bovine Raw Milk with Yersinia enterocolitica Biotypes in Mashhad, Iran. jhehp 2021; 7 (1) :30-34
URL: http://jhehp.zums.ac.ir/article-1-398-en.html
URL: http://jhehp.zums.ac.ir/article-1-398-en.html
1- Graduated Student of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Social Determinants of Health Research Center, Department of Public Health, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Social Determinants of Health Research Center, Department of Public Health, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract: (5932 Views)
Background: Yersiniosis is an important foodborne zoonotic disease worldwide. Gastroenteritis and septicemia are the primary forms of yersiniosis. Yersinia enterocolitica is a causative organism, which is frequently isolated from foods such as milk. This study aimed to evaluate the contamination rate of bovine raw milk with Y. enterocolitica during January-July 2017 and the biotyping of the isolated organisms.
Methods: In total, 100 bovine raw milk samples were collected from Mashhad, Iran. The samples were enriched in peptone sorbitol broth. After alkali treatment, the enriched samples were streaked onto cefsulodin-irgasan-novobiocin agar plates. Suspected colonies of Y. enterocolitica were tested by the amplification of the 16s rRNA gene to confirm the isolates as Y. enterocolitica. The confirmed isolates were biotyped using biochemical tests.
Results: The results showed that 33% of the bovine raw milk samples were contaminated with Y. enterocolitica. Biotype 1A was predominant (26%), while the pathogenic biotypes 1B (6%) and 5 (1%) were also detected.
Conclusion: According to the results, the isolation of pathogenic Y. enterocolitica from bovine raw milk is a public health hazard due to its presence in dairy products.
Methods: In total, 100 bovine raw milk samples were collected from Mashhad, Iran. The samples were enriched in peptone sorbitol broth. After alkali treatment, the enriched samples were streaked onto cefsulodin-irgasan-novobiocin agar plates. Suspected colonies of Y. enterocolitica were tested by the amplification of the 16s rRNA gene to confirm the isolates as Y. enterocolitica. The confirmed isolates were biotyped using biochemical tests.
Results: The results showed that 33% of the bovine raw milk samples were contaminated with Y. enterocolitica. Biotype 1A was predominant (26%), while the pathogenic biotypes 1B (6%) and 5 (1%) were also detected.
Conclusion: According to the results, the isolation of pathogenic Y. enterocolitica from bovine raw milk is a public health hazard due to its presence in dairy products.
Type of Study: Original Article |
Subject:
Food Safety and Hygiene
Received: 2020/12/14 | Accepted: 2021/02/4 | Published: 2021/03/20
Received: 2020/12/14 | Accepted: 2021/02/4 | Published: 2021/03/20
References
1. Souza RA, Imori PF, Falcao JP. Multilocus Sequence Analysis and 16S rRNA Gene Sequencing Reveal That Yersinia Frederiksenii Genospecies is Yersinia massiliensis. Int J Sys Evol Microbiol. 2013; 63: 3124–9. [Crossref] [Google Scholar]
2. Savin C, Martin L, Bouchier C, Filali S, Chenau J, Zhou Z, et al. The Yersinia pseudotuberculosis Complex: Characterization and Delineation of a New Species, Yersinia wautersii. Int J Med Microbiol. 2014; 304: 452–63. [Crossref] [Google Scholar]
3. Bottone EJ. Yersinia enterocolitica: Revisitation of an Enduring Human Pathogen. Clin Microbiol Newsl. 2015; 37(1): 1-8. [Crossref] [Google Scholar]
4. Jamali H, Paydar M, Radmehr B, Ismail S. Prevalence, Characterization, and Antimicrobial Resistance of Yersinia species and Yersinia enterocolitica Isolated from Raw Milk in Farm Bulk Tanks. J Dairy Sci. 2015; 98(2): 798-803. [Crossref] [Google Scholar]
5. Zhang H, Feng S, Zhao Y, Wang S, Lub X. Detection of Yersinia enterocolitica in Milk Powders by Cross-Priming Amplification Combined with Immunoblotting Analysis. Int J Food Microbiol. 2015; 214: 77–82. [Crossref] [Google Scholar]
6. Myers KM, Gaba J, Al Khaldi SF. Molecular Identification of Yersinia enterocolitica Isolated from Pasteurized Whole Milk Using DNA Microarray Chip Hybridization. Mol Cel Probes. 2006; 20(2): 71-80. [Crossref] [Google Scholar]
7. Soltan Dallal MM, Tabarraie A, MoezArdalan K. Comparison of Four Methods for Isolation of Yersinia enterocolitica from Raw and Pasteurized Milk from Northern Iran. Int J Food Microbiol. 2004; 94: 87-91. [Crossref] [Google Scholar]
8. Hanifian S, Khani S. Prevalence of Virulent Yersinia enterocolitica in Bulk Raw Milk and Retail Cheese in Northern-West of Iran. Int J Food Microbiol. 2012; 155: 89-92. [Crossref] [Google Scholar]
9. World Health Organisation (WHO). Food Safety and Food-borne Illness. Geneva; Switzerland.: 2007.
10. Ackers ML, Schoenfeld S, Markman J, Smith MG, Nicholson MA, DeWitt W, et al. An Outbreak of Yersinia enterocolitica O:8 Infections Associated with Pasteurized Milk. J Infect Dis. 2000; 181: 1834–7. [Crossref] [Google Scholar]
11. Barrett NJ. Communicable Disease Associated with Milk and Dairy Products in England and Wales: 1983–1984. J Infect. 1986; 12: 265–72. [Crossref] [Google Scholar]
12. Ghasemikebria F, Khodabakhshi B, Kouhsari H, Sheshpoli MS, Behnampoor N, Livani S, et al. Yersinia enterocolitica in Cases of Diarrhea in Gorgan, Northern Iran. Med Lab J. 2010; 4(1): 26-33. [In Persian]. [Google Scholar]
13. European Food Safety Authority (EFSA). Opinion of the Scientific Panel on Biological Hazards on Monitoring and Identification of Human Enteropathogenic Yersinia spp. EFSA J. 2007; 595: 1–30. [Crossref]
14. Huovinen E, Sihvonen LM, Virtanen MJ, Haukka K, Siitonen A, Kuusi M. Symptoms and Sources of Yersinia enterocolitica Infection: A Case-Control Study. BMC Infect Dis. 2010; 10(1):1-9. [Crossref] [Google Scholar]
15. Bonardi S, Paris A, Bassi L, Salmi F, Bacci C, Riboldi E, et al. Detection, Semiquantitative Enumeration, and Antimicrobial Susceptibility of Yersinia enterocolitica in Pork and Chicken Meat in Italy. J Food Protect. 2010; 73: 1785-92. [Crossref] [Google Scholar]
16. Petsios S, Fredriksson Ahomaa M, Sakkas H, Papadopoulou C. Conventional and Molecular Methods Used in the Detection and Subtyping of Yersinia enterocolitica in Food. Int J Food Microbiol. 2016; 237: 55-72. [Crossref] [Google Scholar]
17. Tavassoli M, Jamshidi A, Movafagh F, Afshari A. Virulence Characteristics of Yersinia enterocolitica Isolated from Dairy Products in the Northeast of Iran. J Hum Environ Health Promot. 2019; 5(2): 72-8. [Crossref] [Google Scholar]
18. Van Damme I, Berkvens D, Botteldoorn N, Dierick K, Wits J, Pochet B, et al. Evaluation of the ISO 10273:2003 Method for the Isolation of Human Pathogenic Yersinia enterocolitica from Pig Carcasses and Minced Meat. Food Microbiol. 2013; 36(2): 170–5. [Crossref] [Google Scholar]
19. Zeinali T, Jamshidi A, Rad M, Bassami M. A Comparison Analysis of Listeria monocytogenes Isolates Recovered from Chicken Carcasses and Human by Using RAPD PCR. Int J Clin Exp Med. 2015; 8(6): 10152-7.
20. Neubauer H, Hensel A, Aleksic S, Meyer H. Identification of Yersinia enterocolitica within the Genus Yersinia. Sys Appl Microbiol. 2000; 23(1): 58-62. [Crossref] [Google Scholar]
21. Ye Q, Wu Q, Hu H, Zhang J, Huang H. Prevalence and Characterization of Yersinia enterocolitica Isolated from Retail Foods in China. Food Control. 2016; 61: 20–7. [Crossref] [Google Scholar]
22. Rahimi E, Sepehri S, Dehkordi FS, Shaygan S, Momtaz H. Prevalence of Yersinia Species in Traditional and Commercial Dairy Products in Isfahan Province, Iran. Jundishapur J Microbiol. 2014; 7(4): e9249. [Crossref] [Google Scholar]
23. Alavi SM, Rahimi E, Tajbakhsh E. Prevalence of Yersinia enterocolitica in Raw Small Ruminant Milk in Shahrekord, Iran. Bulgarian J Vet Med. 2018; 21(3): 364-70. [Crossref] [Google Scholar]
24. Bernardino Varo L, Quinones Ramirez EI, Fernandez FJ, Vazquez Salinas C. Prevalence of Yersinia enterocolitica in Raw Cow’s Milk Collected from Stables of Mexico City. J Food Protect. 2013; 76(4): 694-8. [Crossref] [Google Scholar]
25. Yucel N, Ulusoy H. A Turkey Survey of Hygiene Indicator Bacteria and Yerinia enterocolitica in Raw Milk and Cheese Samples. Food Control. 2006; 17: 383-8. [Crossref] [Google Scholar]
26. Bonardi S, Bruini I, D'Incaub M, Damme IV, Carniel E, Brémont S, et al. Detection, Seroprevalence and Antimicrobial Resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in Pig Tonsils in Northern Italy. Int J Food Microbiol. 2016; 235: 125-32. [Crossref]
27. Tan LK, Ooi PT, Thong KL. Prevalence of Yersinia enterocolitica from Food and Pigs in Selected States of Malaysia. Food Control. 2014; 35: 94–100. [Crossref] [Google Scholar]
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