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1- Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran; Food and Drug Deputy, Zanjan University of Medical Sciences, Zanjan, Iran.
2- Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran.
3- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
2- Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran.
3- Department of Food Safety and Hygiene, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
Abstract: (626 Views)
Olive oil production represents a major economic activity in Iran. The standard industrial extraction process involves three key steps: (i) crushing of olives to rupture cellular structures and release oil, (ii) malaxation to enhance oil yield through gentle mixing of the olive paste, and (iii) separation of oil from the aqueous and solid residues. In the three-phase centrifugation process, the addition of hot water facilitates oil separation but simultaneously generates large volumes of OMWW and solid waste compounds, particularly phenolic substances, making it one of the most environmentally problematic effluents in the agro-industrial sector. Despite these challenges, OMWW is also a rich source of bioactive compounds, including phenolics, tocopherols, and carotenoids, which exhibit antioxidant properties. The valorization of food industry wastes and by-products, therefore, serves a dual purpose: it ensures the effective utilization of waste streams while simultaneously promoting public health. This approach contributes significantly to the development of a sustainable food chain from both environmental and economic perspectives. Polyphenolic compounds recovered from OMWW show great potential for applications in the food, pharmaceutical, and cosmetic industries, thereby reframing OMWW from an environmental burden to a valuable resource. The aim of this review is to investigate various methodologies for the extraction and purification of polyphenolic compounds from wastes and by-products generated during the olive oil production process.
Type of Study: Review Article |
Subject:
Food Safety and Hygiene
Received: 2026/01/11 | Accepted: 2026/03/16
Received: 2026/01/11 | Accepted: 2026/03/16
References
1. Achmon, Y., & Fishman, A. (2015). The antioxidant hydroxytyrosol: Biotechnological production challenges and opportunities. Applied Microbiology and Biotechnology, 99, 1119-1130. [Crossref]
2. AdamskaSzewczyk, A., & Zgórka, G. (2019). Plant polyphenols in cosmetics-a review. European Journal of Medical Technologies, 3(24), 1-10.
3. Barba, F. J., Zhu, Z., Koubaa, M., Sant'Ana, A. S., & Orlien, V. (2016). Green alternative methods for the extraction of antioxidant bioactive compounds from winery wastes and by-products: A review. Trends in Food Science & Technology, 49, 96-109. [Crossref]
4. Bazinet, L., & Doyen, A. (2017). Antioxidants, mechanisms, and recovery by membrane processes. Critical Reviews in Food Science and Nutrition, 57(4), 677-700. [Crossref]
5. Belwal, T., Chemat, F., Venskutonis, P. R., Cravotto, G., Jaiswal, D. K., Bhatt, I. D., . . . & Luo, Z. (2020). Recent advances in scaling-up of non-conventional extraction techniques: Learning from successes and failures. TrAC Trends in Analytical Chemistry, 127, 115895. [Crossref]
6. Bleve, G., Lezzi, C., Chiriatti, M., D’Ostuni, I., Tristezza, M., Di Venere, D., . . . & Grieco, F. (2011). Selection of non-conventional yeasts and their use in immobilized form for the bioremediation of olive oil mill wastewaters. Bioresource Technology, 102(2), 982-989. [Crossref]
7. Carrara, M., Kelly, M. T., Roso, F., Larroque, M., & Margout, D. (2021). Potential of olive oil mill wastewater as a source of polyphenols for the treatment of skin disorders: A review. Journal of Agricultural and Food Chemistry, 69(26), 7268-7284. [Crossref]
8. Cassano, A., De Luca, G., Conidi, C., & Drioli, E. (2017). Effect of polyphenols-membrane interactions on the performance of membrane-based processes. A review. Coordination Chemistry Reviews, 351, 45-75. [Crossref]
9. Castro-Muñoz, R., Yáñez-Fernández, J., & Fíla, V. (2016). Phenolic compounds recovered from agro-food by-products using membrane technologies: An overview. Food Chemistry, 213, 753-762. [Crossref]
10. Çelik, G., Saygın, Ö., & Balcıoğlu, I. A. (2021). Multistage recovery process of phenolic antioxidants with a focus on hydroxytyrosol from olive mill wastewater concentrates. Separation and Purification Technology, 259, 117757. [Crossref]
11. Dali, I., Aydi, A., Stamenic, M., Kolsi, L., Ghachem, K., Zizovic, I., . . . & Delgado, D. R. (2022). Extraction of lyophilized olive mill wastewater using supercritical CO2 processes. Alexandria Engineering Journal, 61(1), 237-246. [Crossref]
12. de Bock, M., Thorstensen, E. B., Derraik, J. G., Henderson, H. V., Hofman, P. L., & Cutfield, W. S. (2013). Human absorption and metabolism of oleuropein and hydroxytyrosol ingested as olive (Olea europaea L.) leaf extract. Molecular Nutrition & Food Research, 57(11), 2079-2085. [Crossref]
13. Dermeche, S., Nadour, M., Larroche, C., Moulti-Mati, F., & Michaud, P. (2013). Olive mill wastes: Biochemical characterizations and valorization strategies. Process Biochemistry, 48(10), 1532-1552. [Crossref]
14. Foti, P., Romeo, F. V., Russo, N., Pino, A., Vaccalluzzo, A., Caggia, C., & Randazzo, C. L. (2021). Olive mill wastewater as renewable raw materials to generate high added-value ingredients for agro-food industries. Applied Sciences, 11(16), 7511. [Crossref]
15. Frascari, D., Rubertelli, G., Arous, F., Ragini, A., Bresciani, L., Arzu, A., & Pinelli, D. (2019). Valorisation of olive mill wastewater by phenolic compounds adsorption: Development and application of a procedure for adsorbent selection. Chemical Engineering Journal, 360, 124-138. [Crossref]
16. Galanakis, C. M. (2012). Recovery of high added-value components from food wastes: Conventional, emerging technologies and commercialized applications. Trends in Food Science & Technology, 26(2), 68-87. [Crossref]
17. Galanakis, C. M. (2018). Phenols recovered from olive mill wastewater as additives in meat products. Trends in Food Science & Technology, 79, 98-105. [Crossref]
18. Herrero, M., Temirzoda, T. N., Segura-Carretero, A., Quirantes, R., Plaza, M., & Ibañez, E. (2011). New possibilities for the valorization of olive oil by-products. Journal of Chromatography A, 1218(42), 7511-7520. [Crossref]
19. Ianni, F., Gagliardi, A., Taticchi, A., Servili, M., Pinna, N., Schoubben, A., . . . & Bruscoli, S. (2021). Exploiting food-grade mesoporous silica to preserve the antioxidant properties of fresh olive mill wastewaters phenolic extracts. Antioxidants, 10(9), 1361. [Crossref]
20. Jerman Klen, T., & Mozetič Vodopivec, B. (2011). Ultrasonic extraction of phenols from olive mill wastewater: Comparison with conventional methods. Journal of Agricultural and Food Chemistry, 59(24), 12725-12731. [Crossref]
21. Kalogerakis, N., Politi, M., Foteinis, S., Chatzisymeon, E., & Mantzavinos, D. (2013). Recovery of antioxidants from olive mill wastewaters: A viable solution that promotes their overall sustainable management. Journal of Environmental Management, 128, 749-758. [Crossref]
22. Klen, T. J., & Vodopivec, B. M. (2012). The fate of olive fruit phenols during commercial olive oil processing: Traditional press versus continuous two-and three-phase centrifuge. LWT-Food Science and Technology, 49(2), 267-274. [Crossref]
23. Kontos, S., Iakovides, I., Koutsoukos, P., & Paraskeva, C. (2016). Isolation of purified high added value products from olive mill wastewater streams through the implementation of membrane technology and cooling crystallization process. Chemical Engineering Transactions, 47, 337-342.
24. Lafka, T. I., Lazou, A. E., Sinanoglou, V. J., & Lazos, E. S. (2011). Phenolic and antioxidant potential of olive oil mill wastes. Food Chemistry, 125(1), 92-98. [Crossref]
25. Le Floch, F., Tena, M., Rıos, A., & Valcarcel, M. (1998). Supercritical fluid extraction of phenol compounds from olive leaves. Talanta, 46(5), 1123-1130. [Crossref]
26. Mahesar, S., Kandhro, A. A., Cerretani, L., Bendini, A., Sherazi, S., & Bhanger, M. (2010). Determination of total trans fat content in Pakistani cereal-based foods by SB-HATR FT-IR spectroscopy coupled with partial least square regression. Food Chemistry, 123(4), 1289-1293. [Crossref]
27. McNamara, C. J., Anastasiou, C. C., O’Flaherty, V., & Mitchell, R. (2008). Bioremediation of olive mill wastewater. International Biodeterioration & Biodegradation, 61(2), 127-134. [Crossref]
28. Mikdame, H., Kharmach, E., Mtarfi, N. E., Alaoui, K., Ben Abbou, M., Rokni, Y., . . . & Rais, Z. (2020). By‐products of olive oil in the service of the deficiency of food antioxidants: The case of butter. Journal of Food Quality, 2020(1), 6382942. [Crossref]
29. Montenegro-Landívar, M. F., Tapia-Quirós, P., Vecino, X., Reig, M., Valderrama, C., Granados, M., . . . & Saurina, J. (2021). Recovery of added-value compounds from orange and spinach processing residues: Green extraction of phenolic compounds and evaluation of antioxidant activity. Antioxidants, 10(11), 1800. [Crossref]
30. Ntougias, S., Bourtzis, K., & Tsiamis, G. (2013). The microbiology of olive mill wastes. BioMed Research International, 2013(1), 784591. [Crossref]
31. Obied, H. K., Bedgood Jr, D., Prenzler, P. D., & Robards, K. (2007). Bioscreening of Australian olive mill waste extracts: Biophenol content, antioxidant, antimicrobial and molluscicidal activities. Food and Chemical Toxicology, 45(7), 1238-1248. [Crossref]
32. Oliveira, J. V., & Oliveira, D. (2000). Kinetics of the enzymatic alcoholysis of palm kernel oil in supercritical CO2. Industrial & Engineering Chemistry Research, 39(12), 4450-4454. [Crossref]
33. Pappas, V. M., Lakka, A., Palaiogiannis, D., Athanasiadis, V., Bozinou, E., Ntourtoglou, G., . . . & Lalas, S. I. (2021). Optimization of pulsed electric field as standalone “green” extraction procedure for the recovery of high value-added compounds from fresh olive leaves. Antioxidants, 10(10), 1554. [Crossref]
34. Paulo, F., & Santos, L. (2021). Deriving valorization of phenolic compounds from olive oil by-products for food applications through microencapsulation approaches: A comprehensive review. Critical Reviews in Food Science and Nutrition, 61(6), 920-945. [Crossref]
35. Peschel, W., Sánchez-Rabaneda, F., Diekmann, W., Plescher, A., Gartzía, I., Jiménez, D., . . . & Codina, C. (2006). An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chemistry, 97(1), 137-150. [Crossref]
36. Pinto, J. N., Pereira, A., Silva, A. R. R., Cardoso, D. N., Mostafaie, A., Campos, F., . . . & Murta, D. (2025). A Comprehensive ecotoxicological evaluation of a treated olive mill wastewater and obtained sludge. Toxics, 13(8), 648. [Crossref]
37. Priego-Capote, F., Ruiz-Jiménez, J., & De Castro, M. L. (2004). Fast separation and determination of phenolic compounds by capillary electrophoresis-diode array detection: Application to the characterisation of alperujo after ultrasound-assisted extraction. Journal of Chromatography A, 1045(1-2), 239-246. [Crossref]
38. Rathee, P., Sehrawat, R., Rathee, P., Khatkar, A., Akkol, E. K., Khatkar, S., . . . & Sobarzo-Sánchez, E. (2023). Polyphenols: Natural preservatives with promising applications in food, cosmetics and pharma industries; Problems and toxicity associated with synthetic preservatives; Impact of misleading advertisements; Recent trends in preservation and legislation. Materials, 16(13), 4793. [Crossref]
39. Rodríguez-Pérez, M., García-Béjar, B., Burgos-Ramos, E., & Silva, P. (2025). Valorization of olive oil and wine industry byproducts: Challenges and opportunities in sustainable food applications. Foods, 14(14), 2475. [Crossref]
40. Roselló-Soto, E., Galanakis, C. M., Brnčić, M., Orlien, V., Trujillo, F. J., Mawson, R., . . . & Barba, F. J. (2015). Clean recovery of antioxidant compounds from plant foods, by-products and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends in Food Science & Technology, 42(2), 134-149. [Crossref]
41. Servili, M., Sordini, B., Esposto, S., Urbani, S., Veneziani, G., Maio, I. D., . . . & Taticchi, A. (2013). Biological activities of phenolic compounds of extra virgin olive oil. Antioxidants, 3(1), 1-23. [Crossref]
42. Słota, P., Harasym, J., & Jacukowicz-Sobala, I. (2025). Supercritical fluid extraction-a sustainable and selective alternative for tannin recovery from biomass resources. Applied Sciences, 15(11), 5914. [Crossref]
43. Tapia-Quirós, P., Montenegro-Landívar, M. F., Reig, M., Vecino, X., Cortina, J. L., Saurina, J., & Granados, M. (2022). Recovery of polyphenols from agri-food by-products: The olive oil and winery industries cases. Foods, 11(3), 362. [Crossref]
44. Teo, C. C., Tan, S. N., Yong, J. W. H., Hew, C. S., & Ong, E. S. (2010). Pressurized hot water extraction (PHWE). Journal of Chromatography A, 1217(16), 2484-2494. [Crossref]
45. Troise, A. D., Fiore, A., Colantuono, A., Kokkinidou, S., Peterson, D. G., & Fogliano, V. (2014). Effect of olive mill wastewater phenol compounds on reactive carbonyl species and Maillard reaction end-products in ultrahigh-temperature-treated milk. Journal of Agricultural and Food Chemistry, 62(41), 10092-10100. [Crossref]
46. Veneziani, G., Novelli, E., Esposto, S., Taticchi, A., & Servili, M. (2017). Applications of recovered bioactive compounds in food products. In Ch. M. Galanakis,Olive mill waste (pp. 231-253). Elsevier. [Crossref]
47. Zagklis, D. P., & Paraskeva, C. A. (2020). Preliminary design of a phenols purification plant. Journal of Chemical Technology & Biotechnology, 95(2), 373-383. [Crossref]
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