en Food Safety and Hygiene Food Safety and Hygiene پژوهشي Original Article <strong>Background</strong>: Yersiniosis is an important foodborne zoonotic disease worldwide. Gastroenteritis and septicemia are the primary forms of yersiniosis. <em>Yersinia enterocolitica </em>is a causative organism, which is frequently isolated from foods such as milk. This study aimed to evaluate the contamination rate of bovine raw milk with <em>Y. enterocolitica </em>during January-July 2017 and the biotyping of the isolated organisms.<br> <strong>Methods</strong>: &nbsp;In total, 100 bovine raw milk samples were collected from Mashhad, Iran. The samples were enriched in peptone sorbitol broth. After alkali treatment, the enriched samples were streaked onto cefsulodin-irgasan-novobiocin agar plates. Suspected colonies of <em>Y. enterocolitica</em> were tested by the amplification of the 16s rRNA gene to confirm the isolates as <em>Y. enterocolitica. </em>The confirmed isolates were biotyped using biochemical tests.<br> <strong>Results</strong>: The results showed that 33% of the bovine raw milk samples were contaminated with <em>Y. enterocolitica</em>. Biotype 1A was predominant (26%), while the pathogenic biotypes 1B (6%) and 5 (1%) were also detected.<br> <strong>Conclusion</strong>: &nbsp;According to the results, the isolation of pathogenic <em>Y. enterocolitica</em> from bovine raw milk is a public health hazard due to its presence in dairy products. Yersinia enterocolitica, Milk, Yersinia, Sample enrichment, 16s rRNA gene, 30 34 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-268-1&slc_lang=en&sid=1 Fahimeh Movafagh movafagh1391@yahoo.com 100319475328460011144 100319475328460011144 No Graduated Student of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Tayebeh Zeinali ta.zeinaly@gmail.com 100319475328460011145 100319475328460011145 No Social Determinants of Health Research Center, Department of Public Health, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran. Abdollah Jamshidi ajamshid@um.ac.ir 100319475328460011146 100319475328460011146 Yes Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.