en Environmental Health, Sciences, and Engineering Environmental Health, Sciences, and Engineering پژوهشي Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong>Background:</strong> The present study aimed to investigate the protective effects of selenium nanoparticles in a lead-induced testicular toxicity model.<br> <strong>Methods</strong>: In total, 30 Wistar rats were divided into three groups and treated (except the normal control) with lead acetate in drinking water (1,000 mg/l) for five weeks. The control rats and negative control rats received saline intraperitoneally, and the third group received intraperitoneal injections of selenium nanoparticles (0.5 mg/kg).<br> <strong>Results: </strong>The lead-treated group showed a significant increase in blood urea nitrogen, serum creatinine, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and malondialdehyde levels (<em>P&nbsp;</em>< 0.01). The lead-intoxicated rats treated with selenium nanoparticles showed a significant decrease in serum AST and ALT compared to the untreated negative controls (<em>P&nbsp;</em>< 0.5). The histopathological examination of liver and kidney tissues indicated lead-induced injuries (e.g., necrotic cells in liver and kidneys). The selenium-treated group showed reduced histopathological signs of lead-induced injuries. Lipid peroxidation levels were also lower in the selenium-treated rats compared to the negative controls (<em>P&nbsp;</em>< 0.05).<br> <strong>Conclusion</strong>: This experimental study confirmed the protective effects of selenium nanoparticles in the rats exposed to chronic lead-induced toxicity. However, further experiments are required to evaluate the possible side-effects and complications.&nbsp;</span></span><gdiv></gdiv> <gdiv></gdiv><gdiv></gdiv></div> Lead acetate, selenium nanoparticles, liver, kidney, Toxicity, 35 40 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-424-1&slc_lang=en&sid=1 Mohammad Reza Hajinezhad hajinezhad@uoz.ac.ir 100319475328460011147 100319475328460011147 Yes Department of Basic Veterinary Science, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.