en Food Safety and Hygiene Food Safety and Hygiene پژوهشي Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:1;"><span style="color:black"><strong>Background:</strong>&nbsp;In the context of sustainable agriculture, reducing chemical fertilizer use is crucial to protect environmental and human health. This study aimed to evaluate the effects of various fertilizer regimes and tuber inoculation with beneficial bacteria on the quality traits of the potato cultivar Sant&eacute;. </span><br> <b><span style="color:black">Methods:</span></b><span style="color:black"> A field experiment was conducted during the 2016-2017 growing seasons at the Agricultural Research Farm of Hamadan Islamic Azad University. Treatments included five fertilizer regimes: no fertilizer (control), 100% nitrogen (N), 66% N + 33% organic fertilizer, 33% N + 66% organic fertilizer, and 100% organic fertilizer. Further, tubers were inoculated with <i>Azospirillum</i>, <i>Pseudomonas</i>, or a combination of both. </span><br> <b><span style="color:black">Results:</span></b><span style="color:black"> In 2017, the lowest nitrate content (16.67 mg/kg) was observed with 33% N + 66% organic fertilizer treatment combined with <i>Pseudomonas </i>inoculation. The highest protein content (7.05%) was recorded under the 100% organic fertilizer treatment with dual bacterial inoculation. The highest starch content (19.76%) and protein yield (1861 kg/ha) were obtained in 2016 under combined fertilizer and bacterial treatments. Yield components improved with partial nitrogen replacement by organic fertilizer,</span> <span style="color:black">particularly when <i>Pseudomonas</i> was applied. </span><br> <b><span style="color:black">Conclusion:</span></b> <span style="color:black">While biofertilizers alone may not fully replace chemical fertilizers, their integration with organic inputs can significantly enhance potato quality and yield while reducing nitrate accumulation. These findings support the adoption of integrated fertilization strategies as a viable approach toward more sustainable potato production systems.</span></span></span></span></div> Azospirillum, Protein, Pseudomonas, Nitrate accumulation, Starch, 244 252 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-681-2&slc_lang=en&sid=1 Ali Akber Naghdi a.naghdi@yahoo.com 100319475328460015954 0009-0008-0857-8823 No Department of Agricultural Sciences and Engineering, SR.C., Islamic Azad University, Tehran, Iran. Saeed Piri saeedpiripiran@gmail.com 100319475328460015955 100319475328460015955 Yes Department of Horticulture Science, Ab.c., Islamic Azad University, Abhar, Iran. Ahmad Khaligi akhalighi@ut.ac.ir 100319475328460015956 0009-0005-3816-681X No Department of Agricultural Sciences and Engineering, SR.C., Islamic Azad University, Tehran, Iran. Pezhman Moradi pjmoradi@yahoo.com 100319475328460015957 100319475328460015957 No Department of Horticultural Science, Sav.c., Islamic Azad University, Saveh, Iran.