en Food Safety and Hygiene Food Safety and Hygiene پژوهشي Original Article <div><span style="line-height:1;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><b><span style="border: 1pt none windowtext; padding: 0in;"><span style="background:white"><span style="color:black">Background</span></span></span></b><strong>:</strong> Today, the prevalence of foodborne illnesses has led to the widespread use of medicinal plants. This study aims to investigate the antibacterial effect of polylactic acid (PLA) films containing oak ethanolic extracts incorporated with cinnamon essential oil against a range of foodborne pathogens.<br> <b><span style="border: 1pt none windowtext; padding: 0in;"><span style="background:white"><span style="color:black">Methods: </span></span></span></b>The oak extract was obtained by soaking method and the cinnamon essential oil was extracted using a Clevenger apparatus. PLA films containing 2% concentration of oak extract and cinnamon essential oil were prepared, homogenized, cast, and stored at 4&deg;C. Subsequently, the antimicrobial efficacy of various concentrations of the extract against pathogenic bacteria was examined using the disk diffusion method.<br> <b><span style="border: 1pt none windowtext; padding: 0in;"><span style="background:white"><span style="color:black">Results: </span></span></span></b>The film containing 400 mg/mL of ethanolic extract along with 3% cinnamon, showed the largest non-growth zone (with a diameter of 31.5 mm against <i>Staphylococcus aureus</i>). Furthermore, the film with a concentration of 100 mg/mL of extract displayed the smallest non-growth zone against <i>Salmonella typhimurium</i>, measuring<i> </i>13.54 mm in diameter.<br> <b><span style="border: 1pt none windowtext; padding: 0in;"><span style="background:white"><span style="color:black">Conclusion: </span></span></span></b>Based on the obtained results, the combination of oak extract and cinnamon essential oil has a strong inhibitory effect against common foodborne pathogens. Consequently, these substances may be useful for enhancing the safety and extending the shelf life of various food products.</span></span></span></div> Foodborne pathogen, Disk diffusion, PLA, Oak, Cinnamon, 138 142 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-628-1&slc_lang=en&sid=1 Kimia Bahraminejad kimia.bahraminejad@gmail.com 100319475328460015738 100319475328460015738 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Ali Misaghi amisaghi@ut.ac.ir 100319475328460015739 100319475328460015739 Yes Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Abolfazl Kamkar akamkar@ut.ac.ir 100319475328460015740 100319475328460015740 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Ali Khanjari khanjari@ut.ac.ir 100319475328460015741 100319475328460015741 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.