en Public Health Public Health پژوهشي Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:times new roman;"><strong>Background:</strong> Neutrophils are the most abundant white blood cells in humans. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. NETs is a new strategy for pathogen response. This study focused on whether LPS induced NETs release <em>in vitro</em> in the HL60 cell line.<br> <strong>Methods: </strong>In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. Trypan blue as vital staining was used for viability. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA.<br> <strong>Results:</strong> Our results showed that 75% NETs was produced by HL-60 differentiated neutrophil cells exposed to 200 ng/ml LPS in 45 minues.<br> <strong>Conclusion:</strong> Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.</span></span></div> Netosis, Neutrophil, HL-60, Differentiation, LPS 138 143 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-178-2&slc_lang=en&sid=1 Ehsan Moghanloo Moghanloo.ehsan@ gmail.com 10031947532846009617 10031947532846009617 No Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Elham Ghorbani E.ghorban69@gmail.com 10031947532846009618 10031947532846009618 No Department of Genetics Campus2, Guilan University of Medical Science, Rasht, Iran. Mohammad Sadegh Beikverdi mbeik_2018@yahoo.com 10031947532846009619 10031947532846009619 No Department of Biology Islamic Azad University Central Tehran Branch, Tehran, Iran. Parisa Badameh p.badameh@gmail.com 10031947532846009620 10031947532846009620 No Department of Microbiology, College of Basic Science and Biology,Varamin Pishva Branch, Islamic Azad University, Varamin, Iran. Soheila Rezaei srezaei@yahoo.com 10031947532846009621 10031947532846009621 No Department of Molecular Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. Ahmad Piroozmand proozmand.ahmad@yahoo.com 10031947532846009622 10031947532846009622 No Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Shahram Teimourian teimourians@gmail.com 10031947532846009623 10031947532846009623 No Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran. Minoo Shahidi m..shahidi@yahoo.com 10031947532846009624 10031947532846009624 No Department of Hematology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. Ahmad Khorshidi akhorshidi342@gmail.com 10031947532846009625 10031947532846009625 Yes Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.