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1- Department of Environment, La.C. Islamic Azad University, Lahijan, Iran.
Abstract: (16 Views)
Background: Bacterial tolerance to heavy metals, commonly expressed as minimum inhibitory concentration (MIC), is often evaluated alongside biosorption capacity. However, the extent to which MIC can reliably predict metal absorption has rarely been quantified. This study investigated the quantitative relationship between MIC and biosorption capacity in native bacterial strains isolated from industrially contaminated soils.
Methods: Twenty morphologically and biochemically distinct strains were isolated. MIC values for lead, zinc, cadmium, and a mixture of the three metals were determined using the broth microdilution method. Biosorption capacity was assessed through triplicate batch experiments, and the amount of metal absorbed by biomass was quantified using ICP-OES. A cell-free abiotic control confirmed that metal recovery without biomass remained below 0.5 ppm. The most efficient strains were identified by 16S rRNA gene sequencing. We analyzed the relationship between MIC and biosorption using Pearson correlation, one-way ANOVA, and regression analysis of log-transformed MIC values.
Results: Biosorption capacity consistently increased with resistance level. All tested systems showed significant positive correlations: the multi-metal mixture (r = 0.895), lead (r = 0.879), cadmium (r = 0.780), and zinc (r = 0.748), all with p < 0.001. Strains with higher MIC values showed significantly greater biosorption. Pseudomonas aeruginosa and Bacillus sp. were the most efficient biosorbing strains.
Conclusion: MIC can serve as a simple and cost-effective criterion for the preliminary selection of promising bacterial biosorbents for heavy-metal bioremediation.
Methods: Twenty morphologically and biochemically distinct strains were isolated. MIC values for lead, zinc, cadmium, and a mixture of the three metals were determined using the broth microdilution method. Biosorption capacity was assessed through triplicate batch experiments, and the amount of metal absorbed by biomass was quantified using ICP-OES. A cell-free abiotic control confirmed that metal recovery without biomass remained below 0.5 ppm. The most efficient strains were identified by 16S rRNA gene sequencing. We analyzed the relationship between MIC and biosorption using Pearson correlation, one-way ANOVA, and regression analysis of log-transformed MIC values.
Results: Biosorption capacity consistently increased with resistance level. All tested systems showed significant positive correlations: the multi-metal mixture (r = 0.895), lead (r = 0.879), cadmium (r = 0.780), and zinc (r = 0.748), all with p < 0.001. Strains with higher MIC values showed significantly greater biosorption. Pseudomonas aeruginosa and Bacillus sp. were the most efficient biosorbing strains.
Conclusion: MIC can serve as a simple and cost-effective criterion for the preliminary selection of promising bacterial biosorbents for heavy-metal bioremediation.
Type of Study: Original Article |
Subject:
Environmental Health, Sciences, and Engineering
Received: 2026/07/12 | Accepted: 2026/08/9
Received: 2026/07/12 | Accepted: 2026/08/9
References
1. Abadi, M., Zamani, A., Khosravi, Y., & Homayouni, S. (2023). Comprehensive risk assessment of heavy metals in topsoil and their risks to human health and ecosystem in Zanjan Mineral Province, Western Iran. Water, Air, & Soil Pollution, 234, 627. [Crossref]
2. Akhavan, A., & Golchin, A. (2024). Health risk assessment of lead-zinc mine tailing in Angoran, Zanjan, Iran. Environmental Quality Management, 33(3), 141-155. [Crossref]
3. Ansari, M. I., Masood, F., & Malik, A. (2011). Bacterial biosorption: A technique for remediation of heavy metals. In I. Ahmad, F. Ahmad, & J. Pichtel, Microbes and microbial technology (pp. 283-319). Springer. [Crossref]
4. Aslam, A., Kanwal, F., Javied, S., Nisar, N., & Torriero, A. A. J. (2025). Microbial biosorption: A sustainable approach for metal removal and environmental remediation. International Journal of Environmental Science and Technology, 22(13), 13245-13276. [Crossref]
5. Ayangbenro, A. S., & Babalola, O. O. (2017). A new strategy for heavy metal polluted environments: A review of microbial biosorbents. International Journal of Environmental Research and Public Health, 14(1), 94. [Crossref]
6. Bruins, M. R., Kapil, S., & Oehme, F. W. (2000). Microbial resistance to metals in the environment. Ecotoxicology and Environmental Safety, 45(3), 198-207. [Crossref]
7. Chellaiah, E. R. (2018). Cadmium (heavy metals) bioremediation by Pseudomonas aeruginosa: A minireview. Applied Water Science, 8, 154. [Crossref]
8. Choi, Y., Koh, J., Cha, S. S., & Roe, J. H. (2024). Activation of zinc uptake regulator by zinc binding to three regulatory sites. Nucleic Acids Research, 52(8), 4185-4197. [Crossref]
9. Collin, S., Baskar, A., Geevarghese, D. M., Ali, M. N., Bahubali, P., Choudhary, R., . . . & Swamiappan, S. (2022). Bioaccumulation of lead (Pb) and its effects in plants: A review. Journal of Hazardous Materials Letters, 3, 100064. [Crossref]
10. Firincă, C., Zamfir, L. G., Constantin, M., Răut, I., Capră, L., Popa, D., . . . & Șesan, T. E. (2024). Microbial removal of heavy metals from contaminated environments using metal-resistant indigenous strains. Journal of Xenobiotics, 14(1), 51-78. [Crossref]
11. Flemming, H. C., & Wingender, J. (2010). The biofilm matrix. Nature Reviews Microbiology, 8(9), 623-633. [Crossref]
12. Freundlich, H. (1906). Über die adsorption in Lösungen. Zeitschrift for Physikalische Chemie, 57, 385-470. [Crossref]
13. Fu, F., & Wang, Q. (2011). Removal of heavy metal ions from wastewaters: A review. Journal of Environmental Management, 92(3), 407-418. [Crossref]
14. Gadd, G. M. (1990). Heavy metal accumulation by bacteria and other microorganisms. Experientia, 46(8), 834-840. [Crossref]
15. Gadd, G. M. (2009). Biosorption: Critical review of scientific rationale, environmental importance and significance for pollution treatment. Journal of Chemical Technology and Biotechnology, 84(1), 13-28. [Crossref]
16. Gadd, G. M. (2010). Metals, minerals and microbes: Geomicrobiology and bioremediation. Microbiology, 156(3), 609-643. [Crossref]
17. Gholami Deljomanesh, E., Hakimi Abed, M., Fataei, E., Shariati Feyzabadi, F., & Imani, A. A. (2025). Classification of water quality in the Shafarood River based on physicochemical parameters and heavy metal concentration using multivariate analysis methods. Journal of Human Environment and Health Promotion, 11(2), 129-137. [Crossref]
18. Gul, I., Adil, M., Lv, F., Li, T., Chen, Y., Lu, H., . . . & Feng, W. (2024). Microbial strategies for lead remediation in agricultural soils and wastewater: Mechanisms, applications, and future directions. Frontiers in Microbiology, 15, 1434921. [Crossref]
19. Gupta, P., & Diwan, B. (2017). Bacterial exopolysaccharide mediated heavy metal removal: A review on biosynthesis, mechanism and remediation strategies. Biotechnology Reports, 13, 58-71. [Crossref]
20. Harrison, J. J., Ceri, H., & Turner, R. J. (2007). Multimetal resistance and tolerance in microbial biofilms. Nature Reviews Microbiology, 5(12), 928-938. [Crossref]
21. Ho, Y. S., & McKay, G. (1999). Pseudo-second order model for sorption processes. Process Biochemistry, 34(5), 451-465. [Crossref]
22. Igiri, B. E., Okoduwa, S. I. R., Idoko, G. O., Akabuogu, E. P., Adeyi, A. O., & Ejiogu, I. K. (2018). Toxicity and bioremediation of heavy metals contaminated ecosystem from tannery wastewater: A review. Journal of Toxicology, 2018, 2568038. [Crossref]
23. Jamal, A., Delavar, M. A., Naderi, A., Nourieh, N., Medi, B., & Mahvi, A. H. (2019). Distribution and health risk assessment of heavy metals in soil surrounding a lead and zinc smelting plant in Zanjan, Iran. Human and Ecological Risk Assessment: An International Journal, 25(4), 1018-1033. [Crossref]
24. Joo, J. H., Hassan, S. H. A., & Oh, S. E. (2010). Comparative study of biosorption of Zn²⁺ by pseudomonas aeruginosa and bacillus cereus. International Biodeterioration & Biodegradation, 64(8), 734-741. [Crossref]
25. Karimzadegan, H., Rahmatian, M., Farhud, D. D., & Yunesian, M. (2008). Economic valuation of air pollution health impacts in the Tehran Area, Iran. Iranian Journal of Public Health, 37(1), 20-30.
26. Langmuir, I. (1918). The adsorption of gases on plane surfaces of glass, mica and platinum. Journal of the American Chemical Society, 40(9), 1361-1403. [Crossref]
27. Marzan, L. W., Hossain, M., Mina, S. A., Akter, Y., & Chowdhury, A. M. M. A. (2017). Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint. Egyptian Journal of Aquatic Research, 43(1), 65-74. [Crossref]
28. Masoumi, F., Khadivinia, E., Alidoust, L., Mansourinejad, Z., Shahryari, S., Safaei, M., . . . & Akbari Noghabi, K. (2016). Nickel and lead biosorption by Curtobacterium sp. FM01, an indigenous bacterium isolated from farmland soils of Northeast Iran. Journal of Environmental Chemical Engineering, 4(1), 950-957. [Crossref]
29. Mathivanan, K., Chandirika, J. U., Srinivasan, R., Charles, P. E., Rajaram, R., & Zhang, R. (2023). Exopolymeric substances production by Bacillus cereus KMS3-1 enhanced its biosorption efficiency in removing Cd²⁺ and Pb²⁺ in single and binary metal mixtures. Environmental Research, 228, 115917. [Crossref]
30. Nies, D. H. (2003). Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiology Reviews, 27(2-3), 313-339. [Crossref]
31. Pande, V., Pandey, S. C., Sati, D., Bhatt, P., & Samant, M. (2022). Microbial interventions in bioremediation of heavy metal contaminants in agroecosystem. Frontiers in Microbiology, 13, 824084. [Crossref]
32. Parizanganeh, A., Hajisoltani, P., & Zamani, A. (2010). Assessment of heavy metal pollution in surficial soils surrounding zinc industrial complex in Zanjan-Iran. Procedia Environmental Sciences, 2, 162-166. [Crossref]
33. Priyadarshanee, M., & Das, S. (2021). Biosorption and removal of toxic heavy metals by metal tolerating bacteria for bioremediation of metal contamination: A comprehensive review. Journal of Environmental Chemical Engineering, 9(1), 104686. [Crossref]
34. Sadeghi Poor Sheijany, M., Shariati, F., Yaghmaeian Mahabadi, N., & Karimzadegan, H. (2020). Evaluation of heavy metal contamination and ecological risk of soil adjacent to Saravan municipal solid waste disposal site, Rasht, Iran. Environmental Monitoring and Assessment, 192, 757. [Crossref]
35. Sadeghi Poor Sheijany, M., Shariati, F., & Yaghmaeian Mahabadi, N. (2025). The effects of heavy metals on enzyme activity and microbial biomass of the soil around the waste disposal site (case study of Saravan, Iran). Soil and Sediment Contamination: An International Journal, 34(6), 1420-1442. [Crossref]
36. Tabak, H. H., Lens, P., van Hullebusch, E. D., & Dejonghe, W. (2005). Developments in bioremediation of soils and sediments polluted with metals and radionuclides-1. Microbial processes and mechanisms affecting bioremediation of metal contamination and influencing metal toxicity and transport. Reviews in Environmental Science and Bio/Technology, 4(3), 115-156. [Crossref]
37. Vijayaraghavan, K., & Yun, Y. S. (2008). Bacterial biosorbents and biosorption. Biotechnology Advances, 26(3), 266-291. [Crossref]
38. Wang, J., & Chen, C. (2009). Biosorbents for heavy metals removal and their future. Biotechnology Advances, 27(2), 195-226. [Crossref]
39. Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173(2), 697-703. [Crossref]
40. Zamani, A., Yaftian, M. R., & Parizanganeh, A. (2015). Statistical evaluation of topsoil heavy metal pollution around a lead and zinc production plant in Zanjan province, Iran. Caspian Journal of Environmental Sciences, 13(4), 349-362.
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