en Food Safety and Hygiene Food Safety and Hygiene پژوهشي Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong>Background:</strong> Antimicrobial compounds have numerous applications, and essential oils could be used in edible films to enhance the food shelf life. This study aimed to assess the inhibitory effects of a chitosan/cellulose nanofiber (CNF) film containing the nanoemulsions of <em>Bunium persicum</em> essential oil (NBPEO) and <em>Trachyspermum ammi</em> essential oil (NTEO) on <em>Escherichia coli</em> O₁₅₇:H₇ inoculated in hamburger during storage&nbsp; (4&deg;C).<br> <strong>Methods:</strong> After inoculation, the hamburger samples were classified into three treatment groups of, control, chitosan containing 7.5% CNF (Ch-CNF), and chitosan containing 7.5% CNF enriched with 1.6% NTEO and 0.8% NBPEO (Ch-CNF-NEO). The samples were preserved at the temperature of 4&deg;C, and the bacterial counts were determined on days zero, three, six, nine, and 12. Data analysis was performed using Bonferroni post-hoc test and repeated measures ANOVA.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br> <strong>Results:</strong> The mean <em>E.coli</em> O₁₅₇:H₇ count significantly decreased in the treatments groups compared to the control group. In addition the Ch-CNF-NEO film exerted the most significant inhibitory effects on the growth of <em>E. coli</em> O₁₅₇: H₇ in the hamburger samples.<br> <strong>Conclusion:</strong> According to the results, the Ch-CNF-NEO film could effectively reduce the growth of <em>E. coli</em> O₁₅₇:H₇ in hamburger. Therefore, Ch-CNF-NEO film could be used to effectively increase the safety of meat products against <em>E. coli</em> O₁₅₇: H₇.</span></span></div> <div> <div><div id="_com_1" uage="JavaScript"></div></div> </div> Trachyspermum ammi, Bunium persicum, Chitosan, Escherichia coli O157:H7, Nanoemulsion, 30 34 http://jhehp.zums.ac.ir/browse.php?a_code=A-10-326-1&slc_lang=en&sid=1 Batool Soltaninezhad bsoltaninezhad@gmail.com 100319475328460010236 100319475328460010236 No Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Saeid Khanzadi Khanzadi@um.ac.ir 100319475328460010237 100319475328460010237 Yes Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Mohammad Hashemi mo_hashemi@hotmail.com 100319475328460010238 100319475328460010238 No Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Mohammad Azizzadeh azizzadeh@gmail.com 100319475328460010239 100319475328460010239 No Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.